In 1969 it was dem onstrated1 that pyruvate kinase binds to Blue Dextran 2000. In a study of the mechanism of this absorption it could be shown that the binding of the enzyme is due to an inter­ action of the enzyme with the blue dye of Blue Dextran 2000 and not with the dextran itself. This property was used for the purification of pyruvate kinase of yeast1 and later on also applied to the ex­...

Clear native electrophoresis and blue native electrophoresis are microscale techniques for the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue native electrophoresis, interferes with in-gel fluorescence detection and in-gel catalytic activity assays. This problem can be overcome by omitting the dye in clear native electrophoresis. However, clear native electr...

Intensely blue dye-azoproteins have been prepared by diazotization and coupling of the highly indiffusible blue dye T-1824, Evans blue, with various serum proteins and egg albumin. The products, whether purified by precipitation with alcohol or by chromatography, have a constant dye-to-protein ratio and tests have shown them to be essentially free from unlinked dye. An extremely diffusible dye,...

Brilliant blue is largely found in ice cream, canned processed peas, packet soups, blue raspberry flavored products, dairy products, sweets and drinks. It is also used in soaps, shampoos, mouthwash and other hygiene and cosmetics applications (Archived from Florida). This study was aimed to investigate the antimicrobial effects of Brilliant blue on Bacillus subtilis as a gram positive bacterial...

We report the simple synthesis and full investigation of a novel heparin binding dye, mallard blue, an arginine-functionalized thionine. This dye binds heparin in highly competitive media, including water with high levels of competitive electrolyte, buffered aqueous solution and human serum. The dye reports on heparin levels by a significant change in its UV-vis spectroscopic profile. Molecular...

A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In additio...

The introduction of sentinel lymph node (SLN) biopsy has brought an innovative approach to the staging of early breast cancer. Up to date two methods have been described: a blue dye-guided lymphatic mapping procedure and a radioactivity guided SLN biopsy technique using a handheld gamma probe. Aim of this study was to test the sentinel lymph node concept which states the histopathological analy...

Trypan blue injected intravenously is bound almost at once by the intercellular connective tissue elements all over the body,-by collagen, reticulin, and elastic fibers. This union of dye and tissue elements is the factor responsible for the early macroscopic blue color and is antecedent to cellular colloidopexic action. Different examples of connective tissue differ among themselves in their a...

The phosphomolybdic acid (PMA) has been used as an adsorbent for the removal of dyes (Methylene blue and Congo red dye) from synthetic aqueous solution. Different factors of the adsorbent, like, contact time, pH of the sorption medium, concentration of the dye solution, were investigated by the batch sorption experiment. The adsorption equilibrium data was best fitted by the Langmuir isotherm m...

Denim jeans, dyed with natural or synthetic indigo, are the most popular garments worn by individuals of all ages and approximately one billion pairs of jeans are produced per annum. Traditionally, denim has been woven with cotton in a warp face twill weave, in which the warp is blue whereas the weft is white. Dyeing is usually performed with pure indigo, or indigo combined with a sulfur dye to...