A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis...

West Nile virus RNA was detected in brain tissue from a horse that died in June 2003 in Nuevo Leon State, Mexico. Nucleotide sequencing and phylogenetic analysis of the premembrane and envelope genes showed that the virus was most closely related to West Nile virus isolates collected in Texas in 2002.

Using sludge at the bottom of Hengshui Lake as samples, and screened out the SJ-9 strain with PHA synthesis capability by enrichment, purification, Nile blue staining, far-infrared spectroscopy and gas qualitative and quantitative analysis. The infrared scanning of SJ-9 bacteria product showed that this product was middle chain PHA. Optimization of SJ-9 fermentation conditions obtained the opti...

Scanning electrochemical microscopy (SECM) is used to probe long-range charge transport (CT) through DNA monolayers containing the redox-active Nile Blue (NB) intercalator covalently affixed at a specific location in the DNA film. At substrate potentials negative of the formal potential of covalently attached NB, the electrocatalytic reduction of Fe(CN)6(3-) generated at the SECM tip is observe...

R E S U L T S A N D D I S C U S S I O N . . . . . . . . . . 3 8 4 O b s e r v a t i o n s o n t i s s u e s . . . . . . . . . . 3 8 4 E x a m i n a t i o n of K a u f m a n n a n d L e h m a n n ' s r e s u l t s . . . . . . 3 8 4 (a) G e n e r a l c o n s i d e r a t i o n s . . . . . . . . 3 8 4 (6) B l u e s t a i n i n g m i x t u r e s c o n t a i n i n g t r i o l e i n . . . . . . . 3 8 ...

The prevalence of West Nile virus (WNV) infections and associated morbidity has accelerated in recent years. Of particular concern is the recent demonstration that this virus can be transmitted by blood products and can cause severe illness and mortality in transfusion recipients. We have evaluated methylene blue (MB)+light as a safe and cost-effective means to inactivate WNV in vitro. This reg...

Serum samples were obtained from 24 horses in the State of Coahuila, Mexico, in December 2002. Antibodies to West Nile virus were detected by epitope-blocking enzyme-linked immunosorbent assay and confirmed by plaque reduction neutralization test in 15 (62.5%) horses. We report the first West Nile virus activity in northern Mexico.

This study was conducted to produce a high percentage of genetically male tilapia through interspecific hybridization between Nile tilapia, Oreochromis niloticus and Blue tilapia, O. aureus and evaluate sex ratio, productive performance and heterosis of the progeny produced. The results revealed that sex ratios of the progenies of (♀ O. aureus x ♂ O. niloticus) and (♀ O. niloticus x ♂ O. aureus...