bordetella pertussis is a gram negative bacterium that causes respiratory tract infection in human (whooping cough). pertussis toxin (pt) is the main component of current acellular pertussis vaccine and the s1 (subunit1) is the main immunogenic part of it. thus, s1 has been the target of many studies as a potent candidate of acellular vaccine against bordetella pertussis, lacking the side effec...

results among the specimens, 11 cases (1.4%) were culture-positive. among these isolates, only two isolates had high mic values for erythromycin and clarithromycin. pulsed-field gel electrophoresis analysis of the isolates revealed 6 pfge profiles (a-f) among which three and two isolates had the same patterns in profiles a and b, respectively. conclusions azithromycin can be a good drug of choi...

bordetella pertussis infects the respiratory tract of the human host and causes whooping cough in children. the nature of immunity against bordetella pertussis infection and disease is poorly understood. the aim of this study was to investigate cell mediated immunity in mice immunized with outer membrane component of cell wall, of b. pertussis. a group of mice were immunized with outer membrane...

The genomes of three closely related bordetellae are currently being sequenced, thus providing an opportunity for comparative genomic approaches driven by an understanding of the comparative biology of these three bacteria. Although the other strains being sequenced are well studied, the strain of Bordetella parapertussis chosen for sequencing is a recent human clinical isolate (strain 12822) t...

background and objectives: bordetella species colonize the respiratory tract of mammals and thereby cause the whooping cough. most of the species produce adenylate cyclase - a toxin ( hemolysin ) responsible for increasing intracellular cyclic amp (camp) levels in mammalian neutrophils and macrophages and as a consequence their phagocytic function get impaired . this study was carried out to is...

Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bordetella bronchiseptica, which do not produce any PT, are shown to have a weaker...

CDC nonoxidizer group 2 (NO-2) currently consists of 15 gram-negative, rod-shaped, oxidase-negative, asaccharolytic, brown soluble pigment-producing strains isolated from blood cultures, usually from young adults. On the basis of their cellular fatty acid profiles, NO-2 strains formed a single group that was identical with the profile of Bordetella avium. 16S rRNA sequencing of one NO-2 strain ...

Genetic and biochemical studies have established that Fur and iron mediate repression of Bordetella alcaligin siderophore system (alc) genes under iron-replete nutritional growth conditions. In this study, transcriptional analyses using Bordetella chromosomal alc-lacZ operon fusions determined that maximal alc gene transcriptional activity under iron starvation stress conditions is dependent on...

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and...