In order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (DTH). A mixture of cytoplasmic proteins from Brucella melitensis B115 was used as a specific antigenic stimulus in bovine whole blood culture. Supernatants harvested at 18 to 24 h after the in vitro antigenic st...

A serological survey of brucellosis in cattle and buffalo was performed in 23 States of India. A total of 30,437 bovine samples, comprising 23,284 cattle and 7,153 buffalo (Bubalus bubalis), were screened. The screening initially used the rose bengal plate test: doubtful and positive samples were then titrated in the serum tube agglutination test. The overall prevalence rate of antibodies was 1...

Bovine Brucellosis is a serious disease of livestock that has significant animal, public health and international trade consequences. Considering the damage done by the infection in animals such as death losses, decreased milk production, weight loss, infertility and lameness, this disease is a formidable threat to livestock. The fact that this disease can spread rapidly and be transmitted to h...

The mandatory bovine abortion notification system in France aims to detect as soon as possible any resurgence of bovine brucellosis. However, under-reporting seems to be a major limitation of this system. We used a unilist capture-recapture approach to assess the sensitivity, i.e. the proportion of farmers who reported at least one abortion among those who detected such events, and representati...

Brucellosis, commonly known as “undulant fever”, “Mediterranean fever” or “Malta fever” is a leading cause of zoonosis worldwide caused by the bacterial genus brucella [1]. Brucella is an aerobic, gram-negative, non-fermenting, facultative intracellular, non-motile, non-spore-forming, cocci, cocobacilli or short rods based on DNA homology and represent a single species [2,3]. It is transmitted ...

BACKGROUND Since 2005, France has been officially free of brucellosis, an infectious disease that causes abortion in cattle and can be transmitted from cattle to humans. Recent animal and human cases have drawn attention to the need to prevent infection of humans and animals from any primary outbreaks. In order to detect any new outbreaks as soon as possible, a clinical surveillance system requ...

A cross-sectional epidemiological study was carried out from September 2004 to March 2005 to determine the seroprevalence and identify risk factors for seropositivity of bovine brucellosis in the extensive cattle production systems of Tigray Region. The study populations comprised indigenous breed cattle in the region, and samples were selected by 2-stage cluster sampling. Serum samples collect...

Brucella abortus, a smooth strain of the genus Brucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid sa...

Bacteriophages are viruses that infect bacteria. Although their effects on bacteria have been known for many years, the discovery of antibiotics has limited widespread use bacteriophages against bacterial infections. However, it is now essential to reconsider using phage therapies due antimicrobial-resistant and uncontrolled zoonotic diseases becoming a global problem. This study aimed isolate ...