The objective of the present study was to assess the capacity of nonsynaptic brain mitochondria to accumulate Ca2+ when subjected to repeated Ca2+ loads, and to explore under what conditions a mitochondrial permeability transition (MPT) pore is assembled. The effects of cyclosporin A (CsA) on Ca2+ accumulation and MPT pore assembly were compared with those obtained with ubiquinone 0 (Ubo), a qu...

Previous work has indicated that two types (A and B) of binding sites for hexokinase exist, but in different proportions, on brain mitochondria from various species. Hexokinase is readily solubilized from Type A sites by glucose 6-phosphate (Glc-6-P), while hexokinase bound to Type B sites remains bound even in the presence of Glc-6-P. Type A:Type B ratios are approximately 90:10, 60:40, 40:60,...

in this study the uptake and metabolism of adenosine by mitochondria has been investigated. incubation of cem cells mitochondria preparation with [3h] -adenosine showed substantial uptake and metabolism of adenosine. adenosine was both anabolized to amp, adp and atp, and also catabolized to inosine. the highest concentration of metabolites in extracted mitochondria was due to amp. the mitochond...