objective(s) in this study, a kind of ph sensitive composite membrane was prepared and drug permeation through it was investigated in terms of ph. rationale of this study originated from the fact that a ph change which may be a result of a disease state in the body can trigger drug release. materials and methods  here, a kind of ph sensitive composite membrane containing different nanoparticle ...

Authors are with the Department of Neurology, University of Wisconsin Medical School, Madison, Wis. Address correspondence to Andrew J. Waclawik, MD, Associate Professor of Neurology, Dept of Neurology, CSC H6/574, UW Medical School, 600 Highland Ave, Madison, Wis 53792; 608.263.7539; fax: 608.265.0172; e-mail: [email protected]. ABSTRACT A 23-year-old patient developed diffuse parest...

BACKGROUND Pernicious anaemia is undeniably associated with vitamin B12 deficiency, but the association between subnormal vitamin B12 concentrations and anaemia in older people is unclear. The aim of this systematic review was to evaluate the association between subnormal vitamin B12 concentrations and anaemia in older people. METHODS Clinical queries for aetiology and treatment in bibliograp...

S UBNORMAL PLASMA ascorbic acid concentration and rapid plasma ascorbate clearance have been found in patients with vitamin B12 deficiency despite adequate intake of vitamin C. 2 Cox et al.2 noted in their group of vitamin B12-deficient patients that these abnormalities of ascorbate metabolism were still present 7-14 days following therapy with sufficient vitamin B12 to cause clinical and hemat...

Two strains of Lactobacillus leichmanii, ATCC 7830 and ATCC 4797, are generally used for vitamin B12 assay. However, the complex growth requirements and the lack of specificity to vitamin B12 of these lactic acid bacteria make the determination of vitamin B12 difficult (Hoffmann et al., 1948; Skeggs et al., 1948; Snell et al., 1948; Capps et al., 1949; Kitay et al., 1949; Peeler et al., 1949; W...

Peptide digestion from proteases is a significant limitation in peptide therapeutic development. It has been hypothesized that the dietary pathway of vitamin B12 (B12) may be exploited in this area, but an open question is whether B12-peptide conjugates bound to the B12 gastric uptake protein intrinsic factor (IF) can provide any stability against proteases. Herein, we describe a new conjugate ...

Short oligonucleotides binding complementary mRNA essential for bacterial growth could be used as antibacterials provided that they are delivered to cells. One promising oligonucleotide is peptide nucleic acid (PNA), a synthetic DNA analog with neutral peptide-like backbone. Unfortunately, bacteria do not import short oligonucleotides, including PNA, from the environment and efficient non-invas...

We report a case of anaphylactic reaction to intramuscular injection of cyanocobalamin. This 52-year-old lady was diagnosed as a case of megaloblastic anemia secondary to dietary vitamin B12 deficiency. She had severe anaphylactic reaction after the parenteral administration of cyanocobalamine. Later she received oral vitamin B12 with no adverse effects. The purpose of this case report is to dr...

PURPOSE To compare the content of bioaccessible folate, vitamin B12, and isoflavones in tofu and tempe, as influenced by soybean variety and food processing, particularly fermentation. PRINCIPAL RESULTS Raw soybeans contained 2207-2671 μg/kg (dry matter) folate, cooked tempe 1493-4143, and cooked tofu 968-1273 μg/kg, the difference was attributed to the fermentation in tempe. Vitamin B12 was ...

Ten (9.3%) of 107 patients with hepatocellular carcinoma had considerably increased serum unsaturated vitamin B12 binding capacity. All 10 were young (mean 12 years), had no serum alpha-fetoprotein, and no underlying cirrhosis; all had a longer survival compared with patients without increased serum unsaturated vitamin B12 binding capacity in the study. Seven of the 10 patients had fibrolamella...