Pathogenic mechanisms responsible for inflammatory bowel disease (IBD) are poorly understood. In an IBD animal model, the oral administration of polysaccharides such as dextran sulfate sodium (DSS) induces colitis, which exhibit several clinical and histological features for IBD. However, pathogenic factors in the development of colitis remain unclear. Therefore, we investigated possible mechan...

The effect of Phaseolus vulgaris isolectin E4 on polyamine concentrations and ornithine decarboxylase activity of proliferating and differentiating Caco-2 cells was investigated. Values of putrescine, spermidine, and spermine in control cells were highest during the early phase of proliferative cell growth and lowest in the stationary phase. Phytohaemagglutinin E4 significantly increased cellul...

Entamoeba histolytica is a tissue-invasive protozoan parasite causing dysentery in humans. During infection of colonic tissues, amoebic trophozoites are able to kill host cells via apoptosis or necrosis, both of which trigger IL-8-mediated acute inflammatory responses. However, the signaling pathways involved in host cell death induced by E. histolytica have not yet been fully defined. In this ...

Açaí, Euterpe oleracea Mart., is a native plant from the Amazonian and rich in several phytochemicals with anti-tumor activities. The aim was to analyze effects of açaí seed oil on colorectal adenocarcinoma (ADC) cells. In vitro analyses were performed CACO-2, HCT-116, HT-29 cell lines. strains treated for 24, 48, 72 h, viability, death, morphology analyzed. Molecular docking evaluate interacti...

Fusobacterium nucleatum (F. nucleatum) plays a critical role in gastrointestinal inflammation. However, the exact mechanism by which F. nucleatum contributes to inflammation is unclear. In the present study, it was revealed that F. nucleatum could induce the production of proinflammatory cytokines (IL-8, IL-1β and TNF-α) and reactive oxygen species (ROS) in Caco-2 colorectal) adenocarcinoma cel...

The Caco-2 assay has achieved wide popularity among pharmaceutical companies in the past two decades as an in vitro method for estimation of in vivo oral bioavailability of pharmaceutical compounds during preclinical characterization. Despite its popularity, this assay suffers from a severe underprediction of the transport of drugs which are absorbed paracellularly, that is, which pass through ...

rosmarinus officinalis l., a medicinal herb from the labiates family, has been reported to be of potential benefit in the treatment and prevention of several diseases. in particular its phenolics have demonstrated protective effects on various types of cancer through several mechanisms. the present study aimed to determine the effects of rosemary phenolic extracts on human cell functions, with ...

Free fatty acids (FFA) and sn-2-monoacylglycerol (sn-2-MG), the two hydrolysis products of dietary triacylglycerol, are absorbed from the lumen into polarized enterocytes that line the small intestine. Intensive studies regarding FFA transport across the brush-border membrane of the enterocyte are available; however, little is known about sn-2-MG transport. We therefore studied the kinetics of ...

Introducción: En la actualidad las bebidas a base de zumo de frutas llevan adicionadas leche, vitaminas y/o minerales con objeto de mejorar sus efectos beneficiosos para la salud mediante el aporte de numerosos compuestos bioactivos que pueden actuar de forma aditiva o sinérgica frente al estrés oxidativo. Objetivos: Evaluar si la adición de hierro, cinc y leche a las bebidas a base de zumo de ...

Achaete scute-like 2 (Ascl2) is the Wnt signaling target, its regulation by other signaling is undefined. Now we demonstrated that CD133+/CD44+ cell population from HT-29 or Caco-2 cells exhibited cancer stem cell (CSC) properties with highly expressed Ascl2, which is related to the Hippo signaling pathway. YAP1 interference in CD133+/CD44+ HT-29 or Caco-2 cells reduced their proliferation, col...