background and purpose: fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. the aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested pcr. materials and methods: sampling was conducted in...

Objective: To compare the diagnostic performance of direct microscopic techniques and culture media for detecting fungal isolates in hair specimens.Study Design: Cross-sectional study.Place Duration Study: The study was conducted at Department Microbiology, Basic Medical Sciences Institute, Karachi, Pakistan, from January 2021 to June 2021.Materials Methods: A total 207 specimens were collected...

Background Entamoeba gingivalis (E. gingivalis) is an anaerobic protozoan that strongly associated with inflamed periodontal pockets. It able to invade the mucosal epithelium of human host, where it can feed on epithelial cells and elicit a severe innate immune response. Unlike other species, considered E. cannot form cysts, because non-infectious protozoan. The lack encystation capability woul...

We have performed a physiological analysis of the effects of high-osmolarity media on gas1Delta cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of t...

This study reports the presence of filamentous fungi (ff) in drinking water including biofilms. Ff are not studied sufficiently in drinking water. Ff were highest in winter and had an indirect relation with other microorganisms. Pathogenic fungi were not observed at the mesophilic temperatures used. Penicillium expansum and P. brevicompactum were observed which may affect biofilms by mycotoxin ...

Of the various components of barley and oats, one constituent of the cereal cell wall, (1→3,1→4)-β-D-glucan (β-glucan), is important to a wide range of interests. Malting barley breeders strive to develop lines that modify well, yielding malts with low soluble β-glucan levels in Congress worts (less than the American Malting Barley Association guidelines of 100–120 ppm) (http:// www.ambainc.org...

Zinc cluster proteins (or binuclear cluster proteins) possess zinc fingers of the Zn(II)2Cys6-type involved in DNA recognition as exemplified by the well-characterized protein Gal4p. These fungal proteins are transcriptional regulators of genes involved in a wide variety of cellular processes including metabolism of compounds such as amino acids and sugars, as well as control of meiosis, multi-...