Frequency of somaclonal variation in embryogenic calli and plants of Olea europaea L cv.Kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic DNA (RAPD) analysis. Radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in OMc medium supplemented with IBA (5 mgL-1) and 2-ip (0.5 mgL-1). Somatic embryogenesis was induced in calli of radic...

Abstract In vitro-derived plants with elevated ploidy levels can display distinguishing features from the they are derived from, especially owing to their indirect regeneration. Genome size affects cell and, thus, plant morphological characteristics. Therefore, stomata traits may be used as a diagnostic feature allowing identification of ploidy. Actinidia chinensis var. deliciosa A. Chev. (A. C...

St. Augustinegrass is a widely used turf and pasture grass in the southern US. Raleigh is a cultivar known for superior cold tolerance than other St. Augustinegrass cultivars. However, its coarse-leaf texture and long internodes are undesirable when planted in home lawns. Mutagenesis by gamma ray irradiation was employed to treat node cuttings and calli for inducing semi-dwarf growth phenotype....

MAX2 (MORE AXILLARY GROWTH2) is involved in diverse physiological processes, including photomorphogenesis, the abiotic stress response, as well as karrikin and strigolactone signaling-mediated shoot branching. In this study, MdMAX2, an F-box protein that is a homolog of Arabidopsis MAX2, was identified and characterized. Overexpression of MdMAX2 in apple calli enhanced the accumulation of antho...

An efficient protocol has been developed for regeneration of complete plants through somatic embryogenesis in H. coronarium. Creamish white, pale yellow and brown calli were obtained on MS medium supplemented with different concentrations of auxins [2, 4-Dichlorophenoxy acetic acid (2, 4-D), Indole-3 acetic acid (IAA) and 1-Naphthylacetic acid (NAA)] after 4 weeks. Creamy white calli developed ...

A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures

Biochemical adaptations and morphological changes are cellular aptitude originated on biotic and abiotic stresses. Polyethylene glycol (PEG) induces drought stress in the nutrient solution. In the present investigation, Caralluma tuberculata calli is exposed to PEG and antioxidative molecules. By increasing the level of antioxidative enzymes (SOD, POD, CAT, APX, and GR), the PEG-stressed calli ...

A system for Egyptian barley (Hordeum vulgare L. cv Giza 123) transformation via biolistic bombardment has been established. Immature embryos 1.0-1.5 mm in length were aseptically isolated from barley plants cultivated in the open field. Embryos were then cultured on callus induction medium with scutellum side up. Scutellum derived calli were bombarded with the plasmid pAHC25, containing the ma...

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l alpha-n...