background and objectives:  haplophyllum canaliculatum isan endemicand endangerediranian plant from rutaceae family. the object of this work was to study the volatile production in established shoot and callus cultures of haplophyllum canaliculatum as well as isolation, identification and sequencing of 18s rrna gene from callus culture. methods: shoot and callus cultures of h. canaliculatum wer...

Background Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materi...

The effects of eight different narrow band-emitting fluorescent lamps (371-750 nm) and four commercial broad band-emitting fluorescent sources upon growth and shoot initiation in tobacco callus (Nicotiana tabacum var. Wisconsin 38) have been characterized. Wavelength and intensity are equally important parameters in determining morphogenic changes. Near ultraviolet light (371 nm) was found to s...

Aim of this study was to investigate the intensity and timing of the ROS formation, lipid peroxidation and expression of antioxidant enzymes as initial responses of calli of Sesamum prostratum (SP) against Fusarium oxysporum f. sesame crude toxin metabolite of varying concentrations. 2,4 dichlorophenoxy acetic acid (2,4-D) / coconut milk combinations were found to be more efficient among differ...

moringaceae, a monogeneric family in capparales (glucosinolate-containing species), includes 14 species. one of them is moringa peregrina (forssk.) fiori., a small tree, which grows in south east of iran. volatile constituents of seed coat and stem of m. peregrina were determined by gc and gc/ms. moreover, extracts of seed and different cultured cells were analyzed by tlc and gc. three volatile...

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which as an antioxidant. Conventional tomato cultivation not optimal due to the susceptibility plants pests and diseases, even though need for lycopene continues increase. Plant tissue culture method can be solution production. Callus Rampai was initiated fro...

Substituted pyrazinecarboxamides markedly influenced production of flavonolignans in Silybum marianum callus and suspension cultures. In this study the effect of two compounds, N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide (1) and N-(3-iodo-4-methylphenyl)-5-tert-butyl-pyrazine-2-carboxamide (2), as abiotic elicitors on flavono-lignan production in callus culture of S. marianum was investigat...

Background and objectives: Haplophyllum canaliculatum is an endemic and endangered Iranian plant from Rutaceae family. The object of this work was to study the volatile production in established shoot and callus cultures of Haplophyllum canaliculatum as well as isolation, identification and sequencing of 18S rRNA gene from callus culture. Methods: Shoot and callus cultures of H. canaliculatum w...

OBJECTIVES This study compared callus formation in distal femur fractures stabilized with locking plates and intramedullary nails to test the hypothesis that locking plates induce less fracture callus than IM nails. DESIGN Retrospective case matched study. SETTING Two orthopaedic trauma centers. PATIENTS 174 distal femur fracture were reviewed to extract cases treated with retrograde IM n...