Refinement of a perfusion technique permitted the simultaneous measurement of cAMP-elicited [3H]cAMP secretion and intracellular [3H]cAMP levels in sensitive D. discoideum amoebae. These data were compared with measurements of the rate of [32P]cAMP synthesis by extracts of amoebae sonicated at different times during the cAMP signaling response. cAMP stimulation of intact cells led to a transien...

Le camp de Septfonds (Tarn-et-Garonne) est l’un des nombreux camps en France ou furent internes, a partir premiers mois de 1939, milliers Republicains espagnols et etrangers qui avaient combattu leurs cotes pendant la guerre d’Espagne, apres leur defaite devant les troupes franquistes repli France. La recherche Genevieve Dreyfus-Armand sur l’histoire ce camp, mars 1939 l’ete 1944, l’interet pre...

Adenosine 3',5'-cyclic phosphorothioate (cAMP-S) is a cyclic AMP (cAMP) analogue which is only slowly hydrolysed by phosphodiesterases of Dictyostelium discoideum. The affinity of cAMP-S to cAMP receptors at the cell surface is only one order of magnitude lower than that of cAMP. cAMP-S can replace cAMP as a stimulant with respect to all receptor-mediated responses tested, including chemotaxis ...

Specificity and versatility in cyclic AMP (cAMP) signalling are governed by the spatial localisation and temporal dynamics of the signal. Phosphodiesterases (PDEs) are important for shaping cAMP signals by hydrolyzing the nucleotide. In pancreatic β-cells, glucose triggers sub-plasma-membrane cAMP oscillations, which are important for insulin secretion, but the mechanisms underlying the oscilla...

AbstractPurpose Therapeutic recreation (TR) summer camps can provide psychosocial benefits for children and young people (CYP) with chronic illness. At present however, there is a lack of recent research in the UK investigating impacts such camps. This study aimed to investigate experiences TR camp CYP illnesses or disabilities. Specifically, it assess both children's parents'...

The regulatory subunits of both isozymes of cAMP-dependent protein kinase bind 2 mol of cAMP/mol of monomer. cAMP dissociation studies indicate similar cAMP binding behavior for each isozyme. Each has two different intrachain cAMP binding components present in approximately equal amounts and the rate of cAMP dissociation is 5- to 10-fold slower from one site (Site 1) than from the other (Site 2...

The paper presents the results of rescue archaeological excavations carried out at site former German forced labour camp KL Jawischowitz, a sub-camp Auschwitz-Birkenau. It discusses historical material related to individual phases use building complex as well architecture and accompanying technical infrastructure. remains connected with two blocks flats infrastructure functioning in years 1942–...

The binding affinities of the diastereoisomers of adenosine 3',5'-(cyclic)phosphorothioate, Sp-cAMP[S] and Rp-cAMP[S], for the cyclic AMP- (cAMP-)binding sites on purified and reconstituted pig heart type II cAMP-dependent protein kinase holoenzyme were determined by measuring the ability of these compounds to displace [3H]cAMP from this enzyme. Sp-cAMP[S], a cAMP agonist, displaced 50% of the ...

Suspensions of 3':5'-cyclic AMP (cAMP)-sensitive cells of Dictyostelium discoideum responded to a cAMP pulse with increased 3':5'-cyclic GMP (cGMP) levels. Under the assay conditions used (2 x 10(8) cells per ml in 10 mM phosphate buffer, pH 6.0) cAMP (5 x 10(-8) M final concentration) increased cGMP levels from 1 pmol per 10(7) cells to 7 pmol per 10(7) cells in 10 sec and basal levels were re...