Since its first isolation from ox heart by Pangborn (1941), cardiolipin has found general application in the sero-diagnosis of syphilis. Mixtures of cardiolipin, lecithin, and cholesterol have replaced the former tissue extracts as antigens in flocculation and complement-fixation tests. International reference preparations of cardiolipin and of lecithin are being distributed under the supervisi...

Cardiolipin is a mitochondrial phospholipid with a characteristic acyl chain composition that depends on the function of tafazzin, a phospholipid-lysophospholipid transacylase, although the enzyme itself lacks acyl specificity. We incubated isolated tafazzin with various mixtures of phospholipids and lysophospholipids, characterized the lipid phase by (31)P-NMR and measured newly formed molecul...

Although many proteins are known to localize in bacterial cells, for the most part our understanding of how such localization takes place is limited. Recent evidence that the phospholipid cardiolipin localizes to the poles of rod-shaped bacteria suggests that targeting of some proteins may rely on the heterogeneous distribution of membrane lipids. Membrane curvature has been proposed as a facto...

UNLABELLED Neuropsychiatric systemic lupus erythematosus (NPSLE) is one of the most difficult manifestations of lupus to diagnose. Measurement of serum brain antibodies has contributed to early diagnosis and management of NPSLE before development of a debilitating disease. We aimed to assess the value of serum anti-ganglioside M1 antibodies in prediction of NPSLE, in comparison to other antibod...

The effect of 3,4-dihydroxybutyl-1-phosphonate on phosphoglyceride metabolism of Escherichia coli strain 8 was examined. The compound inhibits the incorporation of [33P]phosphate into phospholipids. Phosphatidylglycerol accumulation is very strongly inhibited, while phosphatidylethanolamine and cardiolipin accumulation are less significantly affected. Pulse labeling studies using [32P]phosphate...

A thermodynamic model of multicomponent fluid membranes under the influence of lateral forces is described. Membrane components are characterized by their effective molecular areas, molecular charges, and the set of critical demixing coefficients (differential interaction energies) between each of the different species present. This model is useful in the analysis of electric field induced conc...

An ultracentrifugally homogeneous heat-stable polysaccharide preparation free from serologically reactive rabbit testicular tissue antigen, including cardiolipin, was extracted from the Nichols strain of Treponema pallidum, and found to react by complement-fixation with homologous rabbit sera but not with human syphilitic sera. In addition, the reactive "strain-specific" component was found to ...

BACKGROUND Antibodies against cardiolipin and phosphatidylserine (anti-CL and anti-PS) are associated with thrombosis. In contrast, we determined that IgM antibodies against oxidized CL and PS (OxCL and OxPS) and phosphorylcholine (anti-PC) could be protection markers for cardiovascular disease (CVD). METHODS 226 individuals with established hypertension (diastolic pressure>95 mmHg) from the ...

CL (cardiolipin) is a major mitochondrial membrane phospholipid important for the regulation of mitochondrial function. We examined CL de novo biosynthesis and its resynthesis in isolated rat liver hepatocytes prepared 48 h subsequent to two-thirds PHx (partial hepatectomy). The pool size of CL and its de novo biosynthesis from [1,3-(3)H]glycerol were increased 3.3-fold (P<0.05) and 3.1-fold (P...

Brain cytoplasmic cyclic 3':5'-nucleotide phosphodiesterase (EC 3.1.4.17) requires an endogenous Ca2+-binding protein for ful activity. We now show that lysophosphatidylcholine also effectively enhances activator-deficient phosphodiesterase activity. Stimulation by both ligands was immediate and reversible; both rendered the enzyme more thermally labile, decreased the energy of activation, and ...