A study of the phosphorylated sites on the regulatory subunit of type II cyclic AMP-dependent protein kinase is described. The site of autophosphorylation, previously reported (Takio, K., Walsh, K. A., Neurath, H., Smith, S. B., Krebs, E. G., and Titani, K. (1980) FEBS Lett. 114, 83-88), is found to contain the only residue phosphorylated by the catalytic subunit. Endogenous protein-bound phosp...

SpvC is encoded by the Salmonella virulence plasmid. We have investigated the biochemical function of SpvC and the mechanism by which it is secreted by bacteria and translocated into infected macrophages. We constructed a strain carrying a deletion in spvC and showed that the strain is attenuated for systemic virulence in mice. SpvC can be secreted in vitro by either the SPI-1 or SPI-2 type III...

A methodology of phosphopeptide-selective analysis coupled with column-switching HPLC utilizing titania as precolumn media is presented. Phosphopeptides were selectively enriched on titania packing within a protein/peptide mixture without any additional procedure, and analyzed by column-switching high-performance liquid chromatography. First, phospho-compounds were separated from complex mixtur...

Strong epidemiological evidence suggests that slow prenatal or postnatal growth is associated with an increased risk of CVD and other metabolic diseases. However, little is known whether early growth affects postprandial metabolism and, especially, the appetite regulatory hormone system. Therefore, we investigated the impact of early growth on postprandial appetite regulatory hormone responses ...

BACKGROUND Postprandial lipemia is markedly modulated when carbohydrates are added to a fatty meal. The effect of added protein is less known, however, and the data are controversial. OBJECTIVE We investigated the effects of casein added to various fat-rich meals in the absence and presence of oligosaccharides. DESIGN Four different test meals were given to 24 healthy volunteers: 1) fat alo...

The impact of protein structure in model infant milk formulas (IMFs) on digestion was evaluated using an vitro dynamic model. IMFs differed by their whey profile and the heating conditions. Digesta microstructure followed laser light scattering confocal scanning microscopy. Proteolysis kinetics were monitored SDS-PAGE OPA assay peptide release tandem mass spectrometry. gastric digesta depended ...

Historically, milk proteins have been considered only as food proteins for young mammals because they are their principal source of amino acids. They might also have a physiological importance as a source of biologically active peptides. Since 1979, numerous peptides whose sequences and locations are known have been shown to possess various activities including opiate, antihypertensive, immunom...

Fermentation is a promising solution to valorize cheese whey, the main by-product of dairy industry. In Parmigiano Reggiano production, natural whey starter (NWS), an undefined community thermophilic lactic acid bacteria, obtained from previous day residual through incubation at gradually decreasing temperature after curd cooking. The aim this study was investigate effect fermentation regime (s...

Vasopressin is the primary hormone regulating urine-concentrating ability. Vasopressin phosphorylates the UT-A1 urea transporter in rat inner medullary collecting ducts (IMCDs). To assess the effect of UT-A1 phosphorylation at S486, we developed a phospho-specific antibody to S486-UT-A1 using an 11 amino acid peptide antigen starting from amino acid 482 that bracketed S486 in roughly the center...