conclusions pmf-dependent efflux mechanism appears to play an important role in the mdr phenotype of a. baumannii clinical strains. background multidrug-resistant (mdr) strains of acinetobacter baumannii have been increasingly reported as a major cause of nosocomial infections, and have created major therapeutic problems worldwide. objectives the aim of the present study was to evaluate the rol...

In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potent...

The objective was to determine the effects of osmolality on the energy status of testicular spermatozoa of striped bass incubated in a TRIS free base-NaCl medium (pH 8) adjusted to either 300 (T300) or 600 mOsm/kg (T600) with NaCl. High mitochondrial inner transmembrane potential (DeltaPsim) was assessed (flow cytometry) with the mitochondrial probe 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'-tetraet...

Previous studies have shown that the lymphoblastic leukemia CEM cell line is resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis because of a low expression of caspase-8. Bcl-2 inhibitors, BH3I-2' and HA14-1, are small cell-permeable nonpeptide compounds, are able to induce apoptosis by mediating cytochrome c release, and also lead to dissipation of th...

In January 2016, the American Thyroid Association guidelines recommended hemithyroidectomy as initial treatment for smaller (1-4cm) primary thyroid carcinomas, particularly papillary (PTC) and follicular cancer (FTC). This study evaluated association between guideline release surgical procedures patients with PTC/FTC tumors. Patients tumors 1-4cm who underwent or total-thyroidectomy were identi...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 206 No. 5 655–670 www.jcb.org/cgi/doi/10.1083/jcb.201401070 JCB 655 Correspondence to Thomas L. Schwarz: [email protected] Abbreviations used in this paper: CCCP, carbonyl cyanide 3-chlorophenylhydrazone; CMV, cytomegalovirus; DIV, day in vitro; mt-KR, mitochondrial KillerRed; PD, Parkinson’s disease; ROS, reactive ox...

Apoptosis is a cell death pathway that regulates tissue homeostasis, and is often altered in oncogenesis. Autophagy is a lysosome degradation pathway that mediates cellular adaptation in response to stresses. Altered autophagy pathways are proposed to be associated with pathogenesis of neurodegenerative diseases and oncogenesis. The goal of this work is to study the complex link between apoptos...

To measure sigmaB activation in Listeria monocytogenes under environmental or energy stress conditions, quantitative reverse transcriptase PCR (TaqMan) was used to determine the levels of transcripts for the sigmaB -dependent opuCA and clpC genes in strains having null mutations in genes encoding regulator of sigma B proteins (rsbT and rsbV) and sigma B (sigB) and in the L. monocytogenes wild-t...