We used ELISA and flow cytometry to study the binding of prion protein PrP to glycosaminoglycans (GAGs). We found that recombinant human PrP (rPrP) binds GAGs including chondroitin sulphate A, chondroitin sulphate B, hyaluronic acid, and heparin. rPrP binding to GAGs occurs via the N-terminus, a region known to bind divalent cations. Additionally, rPrP binding to GAGs is enhanced in the presenc...

we developed an immunogen to stimulate multivalent immunity against hepatitis b surface antigen (hbsag) and hepatitis b core antigens (hbcag). immune responses specific for both hbsag and hbcag play an important role in controlling the infection. hbsag-specific antibodies mediate elimination of virions at an early stage of infection and prevent the spread of virus. the immunogen was constructed...

conclusions data from this study about scla were not sufficient and further studies are needed; however, the information here suggests that scla could be a candidate for further research on the design of drugs and vaccines against s. pyogenes infections. results the scla protein is present on the cell surface of the cell and has interact with a common ligand by its hypervariable nh2-terminal re...

lentiviral vectors are among the promising viral based-vectors in gene therapy applications, but the efficiency of their targeting needs to be improved. (strept)avidin-biotin adaptor system is a novel approach to modify the lentiviral envelope for better targeting properties. herein, we describe utilization of this adaptor system by designing a candidate envelope protein-bearing biotin acceptor...

knowing that adenoviral vectors could initiate innate immunity, the ability of e1-deleted recombinant adenovirus (ad-e1δ) in induction of b7.1 and il-2 molecules was studied. methods: the expression of green fluorescent protein in c1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

conclusions while the crp value was in patients with acute brucellosis in the current study, the mpv, rdw and leukocyte counts were within the normal range. crp value remains the most valuable inflammatory marker in cases of acute brucellosis. results the follow-up within the year after treatment was examined. the values of age, gender, leukocyte count, c-reactive protein, rdw and mpv were reco...