In their description of the isolation and properties of ceruloplasmin, Holmberg and Laurel1 (1) noted that this intensely blue copper-protein could be decolorized by reducing agents such as ascorbic acid. Although the decoloriaation was “. . . completely reversible in the presence of oxygen, attempts to (restore blue color by oxidation) with potassium ferricyanide were not successful.” They won...

A sensitivemethod for the detection of ceruloplasmin (ferroxidase) in human serums, using Fe(Il) as the substrate, is described. Initial Fe(ll) concentrations of 30 and 120 p.M may be used, depending on the sensitivity of the spectrophotometer, for the determination of the rate of Fe(Ill) -transferrin formation following the oxidation of Fe(lI) by ceruloplasmin (ferroxidase). Advantages of this...

Ia 103 diabetics simultaneous measurements of serum copper and ceruloplasmin were made, together with the determination of glucose, cholesterol and monoamine oxidase. These diabetics were divided ia two groups: 53 had a diagnosis made iess than two years ago, and 50 had diabetes diagnosed for more than 10 years. A contrcil group consisted in 50 normal individuaIs. Copper and ceruloplasmin of di...

We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (-)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-defici...

• Ceruloplasmin hemocompatibility was confirmed by calorimetry and IL-6 ELISA. Partial purification of ceruloplasmin from human serum gave enzyme higher activity. Direct mediated electron transfer bioelectroreduction oxygen achieved. Simplified methodology to evaluate the bioelectrode inflammatory response shown. High-performance autotolerant bioelectrodes should be ideally suited design implan...

We have determined the amino acid sequence of the amino-terminal 67,000-dalton (67-kDa) fragment of human ceruloplasmin and have established overlapping sequences between the 67-kDa and 50-kDa fragments and between the 50-kDa and 19-kDa fragments. The 67-kDa fragment contains 480 amino acid residues and three glucosamine oligosaccharides. These results together with our previous sequence data f...

The delivery of copper to mammary gland and milk and the effects of lactation were examined in rats. Traces of (67)Cu/(64)Cu(II) were injected intraperitoneally or intravenously into virgin rats or lactating rats (2-5 days postpartum), and incorporation into blood, milk, and tissues was monitored. In virgin rats, most of the isotope first entered the liver and kidney. In lactating rats, almost ...

Recent physicochemical(l,2) and immunochemical(3) studies from our laboratory have shown that human ceruloplasmin is a labile protein the properties of which are readily modified by a variety of experimental conditions. Our previous data, however, indicated that crystalline ceruloplasmin was a single protein. This is at variance with results from other laboratories which have shown that chromat...

We have investigated the results of alterations in indices pro-oxidant (conjugated diene and malondialdehyde) antioxidant (superoxide dismutase, ceruloplasmin, catalase) systems guinea pigs’ lungs under conditions immobilization stress. The experiment was conducted on 40 female pigs weighing 0.18-0.20 kg. animals were divided into 4 groups, each contained 10 pigs: I – intact (control), II–guine...

Aceruloplasminemia is a rare autosomal recessive disease in which a mutation leads to the absence or dysfunction of ceruloplasmin. Deficiency of this enzyme leads to the accumulation of iron in various organs; aceruloplasminemia is usually characterized by diabetes, retinal degeneration and neurological disorders. Diagnosis is suspected by the presence of elevated levels of ferritin, anemia, de...