Changes of cell shape resulting from cellular flattening on culture substratum have previously been demonstrated to correlate with mitotic activity of normal animal cells in monolayer cultures. Here, we compared the shapes and proliferation of chick embryo fibroblasts cultured either in multicellular, multilayered sheets extended between glass fibres, or in standard monolayers. Fibroblasts in s...

introduction: today, investigators pay attention to natural fluids (such as follicular and amniotic fluid) to use them as a medium or a supplement. using human amniotic fluid (haf) in some researches, not only improved in vitro development of preimplantation embryos but also significantly increased percentage of pregnancy. on the contrary, gianaroli et al. have claimed there was no significant ...

This study aims to determine the effect of in-ovo injection L-arginine into local chicken eggs on embryo development and chick performance chicken. A total 160 were incubated using semi-automatic incubator with temperature 37-38C relative humidity 55-65%. The injected solution has a concentration 0.5% (m/v). In treatment was divided 4 groups, first without (control), second 0.2 mL (m/v)/egg, th...