Chinese hamster ovary cells have been treated with bleomycin at various stages in the cell cycle. Mitotic cells, which exhibit least survival, show the greatest amount of DNA strand breakage and a marked inhibition of DNA replication in the subsequent S phase. Strand-rejoining experiments suggest that this is primarily due to differences in the amount of damage produced in the DNA but does not ...

Pai was a keynote speaker at the first Korean symposium held at North Park College in Chicago in October 1991. As a child of an immigrant, her experiences are similar to those of many other young Korean Americans who live in a bicultural world of Korean and American cultures. Seung-Hui Cho, the massacre killer at Virginia Polytechnic Institute, was one of the young Korean Americans. He was born...

An efficient base catalysed approach to the synthesis of thiochromans/chromenes from allenylphosphonates (and an allenoate) and substrates having SH/OH and CHO groups at appropriate positions has been developed. Several azo-linked chromenes that are bright red pigments are also synthesized. This methodology involves the domino reactions of Michael addition and subsequent cyclisation by intramol...

An, J. and A. W. Hsie (1993). "Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine-resistant mutants in Chinese hamster ovary cells: The effects of an inhibitor and a mimic of superoxide dismutase." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289(2): 215. http://www.sciencedirect.com/science/article/B6T2C-47PCN7M41/2/3157a48d39359d02b0...

ANTICANCER DRUGS BY USE OF THERMOSENSITIVE AMPHIPHILIC BLOCK COPOLYMER Kenji Kono, Toshiaki Ozawa, Chie Kojima, Atsushi Harada, Shokyoku Kanaoka, Sadahito Aoshima, Kazuo Maruyama, and Yukihito Ishizaka Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 5998531, Japan Department of Macromolecular Science, Graduate S...

The involvement of myeloperoxidase (MPO) in various inflammatory conditions has been the scope of many recent studies. Besides its well studied catalytic activity, the role of its overall structure and glycosylation pattern in biological function is barely known. Here, the N-glycan composition of native dimeric human MPO purified from neutrophils and of monomeric MPO recombinantly expressed in ...

Most voltage-gated K(+) currents are relatively insensitive to extracellular Na(+) (Na(+)(o)), but Na(+)(o) potently inhibits outward human ether-a-go-go-related gene (HERG)-encoded K(+) channel current (Numaguchi, H., J.P. Johnson, Jr., C.I. Petersen, and J.R. Balser. 2000. Nat. Neurosci. 3:429-30). We studied wild-type (WT) and mutant HERG currents and used two strategic probes, intracellular...

Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with...

The bioCRACK process is a promising technology for the production of second generation biofuels. During this process, biomass is pyrolized in vacuum gas oil and converted into gaseous, liquid and solid products. In cooperation with the Graz University of Technology, the liquid phase pyrolysis process was investigated by BDI - BioEnergy International AG at an industrial pilot plant, fully integr...

Treatment of exponentially growing Chinese hamster ovary cells with bleomycin causes a dose-dependent decrease in cell survival due to DNA damage. This lethal effect can be potentiated by the addition of a nonlethal dose of the anticalmodulin drug N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide ( W13 ) but not its inactive analog N-(4-aminobutyl)-2-naphthalenesulfonamide ( W12 ). By preventi...