The hyperthermophilic archaeal sulredoxin from Sulfolobus tokodaii is a water-soluble high-potential Rieske [2Fe-2S] protein with unique pH-dependent redox properties compared with its mesophilic homologues in cytochrome bc1/b6f complexes. The oxidized recombinant sulredoxin has been crystallized by the hanging-drop vapour-diffusion method using 30%(v/v) polyethylene glycol 400, 0.1 M cadmium c...

Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cer...

An automated headspace gas-chromatographic method has been developed for the determination of acetone in the milk, blood and urine of dairy cows. Five ml samples were saturated with 2 g of sodium chloride and equilibrated for 30 min at 90 degrees C in a Hewlett-Packard HP 19395 A automatic headspace sampler. The headspace volatiles were transferred without splitting to a 25 m x 0.3 mm x 0.4 mic...

A range of monocationic and dicationic dioxyalkylglycerol cytofectins have been synthesised possessing methylene and short n-ethylene glycol spacers. The monocationic compounds were found to be effective in transfections when formulated as lipopolyplexes with peptide and DNA components, in particular with shorter PEG head groups which may have less effect on peptide targeting in the ternary com...

Drought is one of the most important natural phenomenon which affects on plant growth. Alfalfa (Medicago sativa L.) is an essential fodder product which has extensive and depth roots system, this advantage enables the plant to tolerate long term drought without any damage to its regrowth process. In order to measure germination percentage and rate, root and shoot growth of 6 alfalfa cultivars (...

The acetoin and diacetyl contents of culture supernatants of Voges-Proskauer-positive "viridans" streptotocci, Klebsiella pneumoniae and Staphylococcus aureus, were determined by a gas liquid chromatographic procedure, in which supernatants were extracted with diethyl ether and diacetyl was measured on columns of 10% (wt/wt) polyethylene glycol 400 (PEG 400) at 73 C. Acetoin was converted to di...

background: nowadays colonoscopy has a very important role in screening colorectal cancers. in addition, adequate bowel preparation is critical to colonoscopy, as a poor preparation may obscure pathology or prevent full colonoscopy. as it is estimated that unsuccessful colonoscopy leads to second procedures in up to 20% of patients, we sought to compare the effects of different doses of polyeth...

background: fusion of dendritic cells (dcs) with melanoma cells could reinforce the antigenicity of tumors as a strategy for the treatment of malignant melanoma. however, the insufficient quantity of dcs and the low fusion efficiency limits the development of such approach. objective: to define the dosage of the stimulating factors as well as the induction condition for the optimal dcs preparat...

Large volumes of Japanese encephalitis (JE) virus propagated in mouse brain can be easily purified by polyethylene glycol 6,000. By using the polyethylene glycol precipitation method, mouse hemoglobin was almost all separated from the viral suspension, and consequently the total amount of nonviral protein in the viral suspension decreased. The recovery of infectivity was about 100%. The removal...

The micellization of F127 (E(98)P(67)E(98)) in dilute aqueous solutions of polyethylene glycol (PEG6000 and PEG35000) and poly(vinylpyrrolidone) (PVP K30 and PVP K90) is studied. The average hydrodynamic radius (r(h,app)) obtained from the dynamic light scattering technique increased with increase in PEG concentration but decreased on addition of PVP, results which are consistent with interacti...