نتایج جستجو برای: cho cell

تعداد نتایج: 1692099  

Journal: :Biopolymers 1968
I L Graves

Native DNA and polysaccharide (CHO) polymers found in an extract of HeLa cellsl1 as well as the DNA and CHO in sea urchin mitochondria1 preparations12 band a t similar buoyant densities in CsC1. Another association of the two polymers is the interaction of heat-denatured HeLa cell DNA (ADNA) with a CHO from the same kind of cells.3 This interaction may possibly be explained on the basis of hydr...

Journal: :American journal of physiology. Lung cellular and molecular physiology 2002
Erik P Lillehoj Beom T Kim K Chul Kim

We reported previously that Muc1 mucin on the epithelial cell surface is an adhesion site for Pseudomonas aeruginosa (Lillehoj EP, Hyun SW, Kim BT, Zhang XG, Lee DI, Rowland S, and Kim KC. Am J Physiol Lung Cell Mol Physiol 280: L181-L187, 2001). The present study was designed to identify the adhesin(s) responsible for bacterial binding to Muc1 mucin using genetic and biochemical approaches. Ch...

Journal: :The Journal of Cell Biology 2001
Sabine Mechtersheimer Paul Gutwein Nancy Agmon-Levin Alexander Stoeck Matthias Oleszewski Svenja Riedle Rolf Postina Falk Fahrenholz Mina Fogel Vance Lemmon Peter Altevogt

The L1 adhesion molecule plays an important role in axon guidance and cell migration in the nervous system. L1 is also expressed by many human carcinomas. In addition to cell surface expression, the L1 ectodomain can be released by a metalloproteinase, but the biological function of this process is unknown. Here we demonstrate that membrane-proximal cleavage of L1 can be detected in tumors and ...

2011
Jennifer Becker Christina Timmermann Tobias Jakobi Oliver Rupp Rafael Szczepanowski Matthias Hackl Alexander Goesmann Andreas Tauch Nicole Borth Johannes Grillari Alfred Pühler Thomas Noll Karina Brinkrolf

Since 1957 Chinese hamster ovary (CHO) cells are used for in vitro cultivation as they require assimilable low sustenance [1]. Today, CHO cell lines represent the most commonly used mammalian expression system for the production of therapeutic proteins and are considered as the mammalian equivalent of E. coli in research and biotechnology [2]. The production of biopharmaceuticals in CHO cells i...

2006
Paul R. Hoban Michael I. Walton Craig N. Robson Paul R. Mohan Judith Godden Ian J. Stratford Paul Workman Adrian L. Harris Ian D. Hickson

Mitomycin C (MMC) is regarded as the prototype bioreductive alkylating agent in clinical use. To elucidate the biochemical basis of MMC resistance, we isolated a drug resistant derivative (designated CHOMMC) of a Chinese hamster ovary cell line (CHO-K1) by exposure to progressively higher concentrations of MMC. CHO-MMC cells exhib ited a 17-fold increase in resistance to MMC and were 33-fold cr...

Journal: :The Journal of clinical investigation 1989
P R Kaesberg W B Ershler J D Esko D F Mosher

Thrombospondin is a 420-kD platelet alpha-granule glycoprotein that binds specifically to heparin. We examined adhesion to thrombospondin of CHO K1 cells and three mutant CHO lines with varying deficiencies in glycosaminoglycan (GAG) synthesis. In an experiment in which the parent line (K1) had 78% adherence to thrombospondin adsorbed to tissue culture plastic, CHO S745 cells, with less than 6%...

Journal: :Journal of virology 1995
S L Kozak D C Siess M P Kavanaugh A D Miller D Kabat

A rat cDNA (rRam-1), which was cloned on the basis that it enables Chinese hamster ovary (CHO) cells to be infected by amphotropic host range murine retroviruses, was recently found to encode a widely expressed Na(+)-phosphate symporter (M. P. Kavanaugh, D. G. Miller, W. Zhang, W. Law, S. L. Kozak, D. Kabat, and A. D. Miller, Proc. Natl. Acad. Sci. USA 91:7071-7075, 1994). CHO cells express the...

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