BACKGROUND & OBJECTIVES The use of epoxy resin membrane as a support for immobilization of enzyme has resulted into improved sensitivity and stability of biosensors for uric acid, ascorbic acid and polyphenols. The present work was aimed to prepare an improved amperometric biosensor for determination of serum cholesterol required in the diagnostics and management of certain pathological conditi...

BACKGROUND The study assessed whether low maternal serum cholesterol during early pregnancy is associated with preterm delivery. PATIENTS AND METHODS It was a prospective observational cohort study involving pregnant women at gestational age of 14-20 weeks over a period of 12 months. Blood samples were obtained to measure total serum cholesterol concentrations and the sera were then analysed ...

A sensitive and accurate potentiometric reaction-rate method for the assay of peroxidase (EC 1.11.1.7) and peroxidase-coupled reactions is described. The technique is based on the peroxidase-catalyzed rupture of the covalent C-F bond in certain organo-fluoro compounds in the presence of H202 and the measurement of liberated F by a fluoride ion-selective electrode. In a study screening 26 organo...

Arterial stiffening is a risk factor for cardiovascular disease, but how arteries stay supple is unknown. Here, we show that apolipoprotein E (apoE) and apoE-containing high-density lipoprotein (apoE-HDL) maintain arterial elasticity by suppressing the expression of extracellular matrix genes. ApoE interrupts a mechanically driven feed-forward loop that increases the expression of collagen-I, f...

AritraChatterjee The cholesterol oxidase enzyme breaks down cholesterol into an intermediate cholest-5-en-3-one, which is then converted to cholest-4-en-3one (Smith and Brooks 1997) . In the present study this mechanism was exploited. The current produced by the release of electrons from the above reaction was used to determine the amount of free cholesterol in the serum sample. The functioning...

An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...

Earlier communications (1, 2) have described preparations derived from rat liver which are capable of oxidizing the terminal methyl groups of the cholesterol side chain to carbon dioxide. The requisite enzymes (cholesterol oxidase) are all present in the mitochondrial fraction of liver homogenates. Unknown factor(s) present in the soluble portion of the homogenate (supernatant factor), must be ...

Ia 103 diabetics simultaneous measurements of serum copper and ceruloplasmin were made, together with the determination of glucose, cholesterol and monoamine oxidase. These diabetics were divided ia two groups: 53 had a diagnosis made iess than two years ago, and 50 had diabetes diagnosed for more than 10 years. A contrcil group consisted in 50 normal individuaIs. Copper and ceruloplasmin of di...

Serum from a patient with Cushing's syndrome who was being treated with mitotane contained components that interfered with determination of cholesterol in the Du Pont aca. A measured concentration of cholesterol of 4.19 g/L in the undiluted serum increased to a calculated concentration of 9.50 g/L in diluted serum. Adding additional cholesterol oxidase (EC 1.1.3.6) overcame the reaction inhibit...

We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measurements and calculations. In the procedure, cholesterol oxidase oxidizes free chol...