Targeted mass spectrometry comprises a set of methods able to quantify protein analytes in complex mixtures with high accuracy and sensitivity. These methods, e.g., Selected Reaction Monitoring (SRM) and SWATH MS, use specific mass spectrometric coordinates (assays) for reproducible detection and quantification of proteins. In this protocol, we describe how to analyze, in a targeted manner, dat...

Phosphoglyceride phosphorus is readily determined directly on silica gel removed from thin-layer chromatograms, without elution, by a nondigestive procedure with a sulfuric-periodic acid reagent. The method is specific for hydrolyzable phosphoglyceride containing two acid-hydrolyzable groups. Cariolipin reacts only partially, while sphingomyelin, diether lecithin, and phosphonate cephalin fail ...

The recent discovery of activating somatic mutations in cancer that correlate with phenotypes such as drug responsiveness, has generated renewed interest in the sequencing of genomes of tumor samples and cancer cancer cell lines with the goal of identifying the set of mutations that produce those phenotypes [1]. The two most popular strategies for discovering these events are array CGH [2], and...

Abstract. Environmental samples typically contain hundreds or thousands of unique organic compounds, and even minor components may provide valuable insight into their sources transformations. To understand atmospheric processes, individual are frequently identified quantified using gas chromatography–mass spectrometry. However, due to the complexity variable nature such data, data reduction is ...

in order to investigate the medicinal species from montain of shirkooh (up village), in yazd provience, iran on three species by scientific names were heracleum persicum desf. ex fisch., c.a.mey. & avé-lall.; ziziphora clinopodioides lam. and nepeta asterotricha rech.f. were collected on june, july and august 2011. then all samples essential oils were extracted by hydro-distillation method (cla...