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The search for sources from which small changes have an in ̄uence on the response of an analytical system, the ruggedness test, has become an essential part of a validation study. However, the `de®nition' of ruggedness and how to perform a ruggedness test are still ambiguous. In this paper a strategy is proposed to perform a ruggedness test for chromatographic methods. Because the strategy is ba...

Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. Afte...

A gas chromatographic method for the simultaneous analysis of halothane and nitrous oxide in operating theatre atmospheres has been developed and evaluated. The flame ionization detector is suitable for the quantitative analysis of halothane in concentrations approaching one part per million. The frequency-modulated electron capture detector is highly sensitive to nitrous oxide but we have foun...

A potentiometric titration method for the determination of minoxidil based on its redox reaction with K2Cr2O7 is described. The best results were observed using 1.00 x 10-3 mol L-1 K2Cr2O7 and 1.00 x 10-2 mol L-1 minoxidil solutions, and the minoxidil as titrant in 2.00 mol L-1 H2SO4 medium. The method was applied to commercial samples and compared with the results from a chromatographic proced...

A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavele...

Snake bite is crucial medical emergency while the success of immediate care and anti-venom therapy mainly depend on the correct identification of culprit snake. We present a simple method for identification of six snake species including Echiscoloratus, Echispyramidum, Cerastescerastesgaspreti, Bitisarieatans, Najahaje Arabica, andWalterinnesiaaegyptia, using the gel filtration chromatographic ...

Alcoholic fermentation of fruits and cereals is the primary source of alcoholic beverages production. The main component in these drinks is ethanol; but there are also many secondary products like glycerin or acetaldehyde, and mixtures of other higher-order alcohols called “fusel”, also known as congeners. They are important for the characterization of alcoholic beverages and also for forensic ...

The various Mr fractions of polyethylene glycol (PEG) in human urine are quantified by "high-pressure" liquid chromatography. A simple preparation step involving lyophilization and chloroform extraction of the sample is required. Aliquots (10 microL) are chromatographed isocratically in equivolume mixtures of methanol and water on a column of styrene divinylbenzene and the refractive index of t...

Methods: An enantioselective reversed phase high performance liquid chromatographic method was developed and validated. The enantiomers of rivaroxaban was resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 mm) column using a mobile phase system containing n-hexane –isopropanol (50: 50 v/v/) and column temperature at 35°C. The resolution between the enantiomers was not less than 2.0. The developed...