Multidrug-resistant Vibrio cholerae O1 El Tor strains isolated during the 1994 outbreak of cholera in Albania and Italy were characterized for the molecular basis of antibiotic resistance. All strains were found to be resistant to tetracycline, streptomycin, spectinomycin, trimethoprim, sulfathiazole, and the vibriostatic compound O/129 (2,4-diamino-6,7-diisopropylteridine). Resistance genes we...

Screening by a double-disk synergy test identified a Pseudomonas aeruginosa isolate that produced a clavulanic acid-inhibited expanded-spectrum beta-lactamase (ESBL). Cloning and sequencing identified a novel ESBL, BEL-1, weakly related to other Ambler class A ESBLs. beta-Lactamase BEL-1 hydrolyzed significantly most expanded-spectrum cephalosporins and aztreonam, and its activity was inhibited...

conclusions findings of this study showed that integron can have a limited frequency in s. aureus isolated from clinical sample in tehran. results among the 200 samples, 1% of the strains (two isolates) contained the class 1 integron gene. the results of this study showed that the highest frequency of the obtained samples belonged to males and the isolates occurred mostly in individuals aged 51...

Recently another MITE-like structure, called the integron mobilization unit (IMU), has been described flanking a defective class 1 integron harbouring a carbapenem resistance gene, blaGES-5, in a clinical Enterobacter cloacae strain. Although the IMU sequence is not related to the MITE reported in our study, IMU-based mobilization of the integron could be demonstrated using a transposase provid...

An NDM-1 carbapenemase-producing Pseudomonas aeruginosa isolate was recovered from a patient hospitalized in France after a previous hospitalization in Serbia. Genetic studies revealed that the blaNDM-1 gene was surrounded by insertion sequence ISAba125 and a truncated bleomycin resistance gene. This blaNDM-1 region was a part of the variable region of a new complex class 1 integron bearing IS ...

The present work reports the detection of the first case of nosocomial Klebsiella oxytoca producing class A carbapenemase KPC-2 in Brazil. The isolate KPN106 carried a 65-kb IncW-type plasmid that harbors the blaKPC gene and Tn4401b. Moreover, we detected the presence of a class 1 integron containing a new allele, arr-8, followed by a 5'-truncated dhfrIIIc gene. In view of the recent results, w...

Antibiotic resistant E. coli in the animal gut can promote transfer of antibiotic resistance between and human. In order to enforce National Action Plan Bangladesh, it is imperative detect presence harbouring Class 1 integrons that potentially genes among bacteria. Previously used primers pairs were a multiplex PCR successfully species-specific amplicon integron 57% isolates. Plasmid was absent...

Amplification of the gene cassettes in class 1 integrons by PCR using primers in the 5 conserved segment (5 -CS) and the 3 -CS (7, 8) has been used in hundreds of studies to identify integron-associated resistance genes (Fig. 1A). Equivalent PCR primers that detect cassettes in class 2 integrons, i.e., Tn7 family transposons (18), are also widely used. The approach used to detect integrons in a...