The occurrence of multiple herbicide resistant weeds has increased considerably in glyphosate-resistant soybean fields Brazil; however, the mechanisms governing this resistance have not been studied. In its study, target-site and nontarget-site were characterized an Eleusine indica population (R-15) with to acetyl-CoA carboxylase (ACCase) inhibitors, glyphosate, imazamox, paraquat. Absorption t...

The intracellular concentration of free unbound acyl-CoA esters is tightly controlled by feedback inhibition of the acyl-CoA synthetase and is buffered by specific acyl-CoA binding proteins. Excessive increases in the concentration are expected to be prevented by conversion into acylcarnitines or by hydrolysis by acyl-CoA hydrolases. Under normal physiological conditions the free cytosolic conc...

A high molecular weight protein (approximately 1.5--2 x 10(6) daltons) has been found in rat liver cytosol to inhibit acetyl CoA carboxylase activity. The protein inhibitor was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography and gel filtration. The inactivation of the carboxylase is not attributable to either phosphorylation of the enzyme or to action on substrates or ...

Andrimid is a hybrid nonribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-CoA carboxylase (ACC) and thereby inhibits fatty acid biosynthesis with submicromolar potency. The andrimid biosynthetic gene cluster from Pantoea agglomerans encodes an admT gene with homology to the acetyl-CoA carboxyltransferase (CT) beta-subunit gene accD. Escherich...

Biotin is a water-soluble vitamin required by all organisms, but only synthesized by plants and some bacterial and fungal species. As a cofactor, biotin is responsible for carbon dioxide transfer in all biotin-dependent carboxylases, including acetyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and pyruvate carboxylase. Adding biotin to carboxylases is catalyzed by the enzyme holocarboxylas...

The cellular content of acetyl-CoA carboxylase [acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] in Saccharomyces cerevisiae is reduced by the addition of long-chain fatty acids to the culture medium. Mutant strains of S. cerevisiae defective in acyl-CoA synthetase [acid:CoA ligase (AMP-forming), EC 6.2.1.3] were isolated and used to determine whether fatty acid itself or a metabolit...

Plasma insulin concentrations in fed rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in adipose tissue and liver were estimated from the incorporation of 3H from 3H2O. In the adipose tissue dehydrogenase and acetyl-CoA carboxylase were evident. In liver, although changes in rates of fatty acid synthesis were found, the initial activity...

Mycobacterium tuberculosis H37Ra and M. bovis BCG produce multiple methyl-branched fatty acids called mycocerosic acids, presumably from methylmalonyl coenzyme A (CoA). An acyl-CoA carboxylase was isolated from these organisms at a 30 to 50%fo yield by a purification procedure involving ammonium sulfate fractionation, gel ifitration, and affinity chromatography with a monomeric avidin-Sepharose...