The EVIGENE MRSA Detection Kit was evaluated on coagulase-negative staphylococci (CoNS) from agar plates and on staphylococci directly from positive spiked blood cultures. For the CoNS study, a total of 242 isolates were tested, and of these 237 gave valid test results. For the 237 valid tests, all gave correct mecA classification. For the blood culture procedure, a collection of 51 mecA-positi...

The present study aimed to detect Staphylococcus aureus (S. aureus) among other coagulase positive staphylococci from animal origin by using conventional methods (biochemical tests and latex agglutination) and a molecular method, based on the nuc gene, as the gold standard and to assess the usefulness of these methods. For this purpose, total of 344 staphylococcal isolates were collected and an...

Bacteriological quality of individually quick frozen (IQF) shrimp products produced from aquacultured tiger shrimp (Penaeus monodon) has been analysed in terms of aerobic plate count (APC), coliforms, Escherichia coli, coagulase-positive staphylococci, Salmonella, and Listeria monocytogenes. Eight hundred forty-six samples of raw, peeled, and deveined tail-on (RPTO), 928 samples of cooked, peel...

Sanitary conditions are essential for the production of meals and control of the presence of pathogensis important to guarantee the health of customers. The aim of this study was to evaluate the sanitary quality of food services by checking the presence of thermotolerant coliforms, Staphylococcus sp. and evaluate the toxigenic potential from the latter. The analysis was performed on water, surf...

A total of 187 isolates from several clinical specimens were identified to species level as 129 Staphylococcus aureus strains and 58 coagulase-negative staphylococci (CNS) strains by the API Staph System (Biomerieux). Slime production was detected both by the conventional Christensen's method as well as by the Congo red agar method. Seventy-two strains of staphylococci isolates (38.5%) were fou...

Methicillin-resistant staphylococci (MRS), and methicillin-resistant Staphylococcus aureus (MRSA) in particular, have become a public and veterinary health concern. The search for MRS reservoirs outside human hospitals is needed in order to understand the reasons for their persistence and to control their spread. MRS have been isolated from rats, but little is known about their occurrence in fo...

In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococca...

P. pestis AVG2 .... . 0.0 2.85 0.0 2.47 OX/AVG2 ........0.0 1.91 0.0 1.98 Siam ......... 0.0 2.14 0.0 OX/Siam.. 0.0 2.44 0.0 M23 .... 0.0 1.90 0.0 3.43 OX/M23..... 0.0 3.50 0.0 2.50 Yokohama.... 0.0 4.22 0.0 A12........... 0.0 1.78 0.0 Java......... 0.0 1.65 0.0 2.51 TRU .......... 0.0 1.78 0.0 2.29 P. pseudotutberculosis PBI/+.. 0.76 0.50 2.91 2.90 PBI/-.. 2.96 2.12 1.95 2.72 PTB J(2). 1.54 1....

Ice cream is a frozen dairy dessert consumed worldwide. The state may give positive impression regarding microbiological safety; however, transmission of foodborne pathogens can also occur through ice consumption. A total 200 samples milk-based industrial cream, with and without inclusions, were purchased at different mass-market retailers in Italy analyzed for the detection enumeration aerobic...