This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected...

INTRODUCTION The increasing microbial resistance against antibiotics complicates the therapy of bacterial infections. Therefore new therapeutic options, particularly those causing no resistance, are of high interest. Cold atmospheric plasma is one possible option to eradicate multidrug resistant microorganisms, and so far no resistance development against physical plasma is known. METHOD We t...

Nitrous oxide (N2O) has been recognized as a relatively strong greenhouse gas. Heterogeneous catalytic processes have been mainly used to decompose N2O at high temperature. Characteristics of N2O decomposition by catalyst-assisted cold plasma were investigated in the present study. Tubular quartz reactor with annular-shaped electrodes was prepared and ruthenium catalysts supported on alumina pe...

this study was conducted to evaluate the effect of egg yolk and cooling on ram coated spermatozoa. semen was collected from three ram by artificial vagina contacted with a tube containing tris- fructose-egg yolk 15%. samples were pooled, centrifuged by 700 g for 10 min and removed supernatant. then, samples were diluted by tris-glucose and centrifuged again to remove seminal plasma and egg yolk...

OBJECTIVE Acute stress in patients with rheumatoid arthritis (RA) should stimulate a strong stress response. After cryotherapy, we expected to observe an increase of hormones of the adrenal gland and the sympathetic nervous system. METHODS A total of 55 patients with RA were recruited for whole-body cryotherapy at -110 degrees C and -60 degrees C, and local cold therapy between -20 degrees C ...