The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.

The mixing of graphene oxide and protonated poly(m-phenylenediamine) (PmPD) nanoparticles generates floccus that can be readily transformed into graphene@PmPD hybrid hydrogel through redox reaction. This macroscopic material can be directly applied as a monolith column adsorbent for water purification.

the snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. purification and partial characterization of a thrombin-like enzyme from the venom of the iranian snake, agkistrodon halys, was the aim of this study. purification was carried out by a combination of variety of chromatographic methods that included: gel fil...

diphtheria is a fatal disease caused by exotoxin of corynebacterium     diphtheria .  this toxin consists of   two chains, catalytic chain (a) and binding (b) chain. by binding chain (b),   the toxin binds to its receptor on numerous body cells such as myocardial,   kidney and peripheral nerve cells. after entering, catalytic chain (a)   inhibits protein synthesis and finally can cause cell dea...

This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.

The purification of the lignin peroxidase from the juice of Musa paradisiaca stem using a simple procedure involving concentration by ultrafitration and anion exchange chromatography on DEAE cellulose column has been reported.The enzymatic properties of the purified enzyme have been found to be similar to the enzymes reported from the fungal sources.

Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.

A series of arylidine barbituric acid derivatives was synthesized by Knoevenagel condensation reaction of aromatic aldehydes with barbituric acid or thiobarbituric acid using dodecylbenzenesulfonic acid as a Brønsted acid-surfactant catalyst in aqueous media. Regardless of the nature of the substitution (electron-donating and -withdrawing), all the reactions were completed within 15-65 min in w...

Cohen's method for preparing the nerve growth factor from mouse submaxillary glands was followed to the last ammonium sulfate fraction. Further purification was accomplished with carboxymethyl- and diethylaminoethyl- column chromatography. Three peaks were obtained for each column; the third peak obtained with diethylaminoethyl cellulose was the most active. Electrophoresis of this active fract...

We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle and body acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 column followed by several HPLC runs on C18 column using different conditions. Anti-Micrococcus lysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified molecule fr...