Based on the analysis of its genome sequence, the ectomycorrhizal (ECM) basidiomycetous fungus Laccaria bicolorwas shown to be lacking many of the major classes of secreted enzymes that depolymerize plant cell wall polysaccharides. To test whether this is also a feature of other ECM fungi, we searched a survey genome database of Amanita bisporigera with the proteins found in the secretome of Tr...

BACKGROUND Efficient conversion of lignocellulosic biomass to fermentable sugars requires the synergistic action of multiple enzymes; consequently enzyme mixtures must be properly formulated for effective hydrolysis. The nature of an optimal enzyme blends depends on the type of pretreatment employed as well the characteristics of the substrate. In this study, statistical experimental design was...

UNLABELLED BACKGROUND Thermophilic fungi have attracted increased interest for their ability to secrete enzymes that deconstruct biomass at high temperatures. However, development of thermophilic fungi as enzyme producers for biomass deconstruction has not been thoroughly investigated. Comparing the enzymatic activities of thermophilic fungal strains that grow on targeted biomass feedstocks ...

The development biofuels from Lignocellulosic (LC) biomass, which includes a variety of plant feedstocks such as agricultural and forestry residues and dedicated energy crops, is one route to clean and renewable alternatives to petroleum-base fuel. The first step in converting plant biomass to fuels is breaking down cellulose, the most abundant plant cell wall polymer, into fermentable sugars. ...

Background The discovery of lignin as activator for the redox enzyme lytic polysaccharide monooxygenases (LPMOs) for the oxidation of cell-wall polysaccharides opens a new scenario for investigation of the interplay between different lignocellulose-degrading enzymes. The lignin-active enzymes in one hand, and the carbohydrate active in the other, are linked through a variety of electrons carrie...

Beta-aminoisobutyric acid (BAIBA) and branched-chain amino acids (BCAA) have recently received considerable attention because of their various health benefits. In this study, the hydrolysis characteristics chicken breast for 11 commercially proteolytic enzymes were studied to evaluate effect protease type, reaction pH (5-8), leaction time (2-24 h) enzyme concentration (0.25-8%), two combined pr...

BACKGROUND Enzymes for plant cell wall deconstruction are a major cost in the production of ethanol from lignocellulosic biomass. The goal of this research was to develop optimized synthetic mixtures of enzymes for multiple pretreatment/substrate combinations using our high-throughput biomass digestion platform, GENPLAT, which combines robotic liquid handling, statistical experimental design an...

Although sorghum is widely cultivated in Africa, its utilisation for the production of lager type beer by breweries relatively limited. Sorghum used unmalted as a brewing adjunct, limited quantities, with barley and commercial exogenous enzymes production. The use malt sole raw material beers generally because low diastatic power high tannin content among other factors. objective this study to ...