A large proportion of high titre Coxsackie B5 complement-fixation tests was detected among sera giving false positive Wassermann reactions during 1969. Echo virus type 9 proved to be the prevailing enterovirus and is one of those which induces the formation of antibody to Coxsackie B5. One case of Echo virus type 9 meningitis is presented which produced a false positive Wassermann reaction and ...

containing a large amount of neutral sugars showed no complement fixation inhibition. while a O.5N HCI eluate containing proteins alone and one containing proteins and hexosamine showed a slight complenient fixation inhibition, The remaval of lipids from the type specific antigen by hydrolyzing with 1 N HCI for 4 minutes, followed by dialysis, revealed a very strong complement fixation inhibiti...

vorax strain V,. Serological relationships were determined by three procedures: immobilization, agglutination, and complement fixation. Antigenic relationships in general were dependent upon the method employed. The immobilization test provided the best method for uncovering these differences. The complement fixation test did not provide satisfactory identification of the different species.

The phenomenon of conglutination was first reported by Ehrlich and Sachs (1902), and subsequently received attention from Bordet and Gay (1906) and Bordet and Streng (1909). However, a systematic study of the mechanisms involved did not appear until Coombs and associates, in a commendable series of papers, reviewed the earlier work (Hole and Coombs, 1947a), applied the test to certain virus sys...

Ten temperature-sensitive mutants of simian virus 40 have been isolated and characterized in permissive cells. The mutants could be divided into three functional groups and two complementation groups. Seven mutants produced T antigen, infectious viral deoxyribonucleic acid (DNA), and structural viral antigen but predominantly the empty shell type of viral particles. Two mutants produced T antig...

Two methods of performing serological screening tests for syphilis are compared. One consisted of the Venereal Diseases Reference Laboratory (VDRL) slide test, the cardiolipin Wassermann reaction (CWR), and the Reiter protein complement fixation test (RPCFT) performed manually; the other was a fully automated system using two Technicon AutoAnalyzers (AAII), one for the automated reagin test (AR...

Modification of the Microtiter reading mirror used in the standardized diagnostic complement fixation method permits convenient estimation of the results in per cent hemolysis by direct visual comparison with the hemolytic standards.

A complement fixation test modified by the addition of porcine serum and an indirect hemagglutination test were used to detect antibodies to Pseudomonas pseudomallei in pigs. These tests together with cultural examinations were carried out with 250 pigs. The sensitivity and specificity values were 79.3 and 99.5% and 82.8 and 93.2% for the modified complement fixation and hemagglutination tests,...

False positive raised complement fixation titres to egg-grown virus preparations were found in six pigeon fanciers suffering from extrinsic allergic alveolitis but not in six similar budgerigar fanciers. The raised titres in the pigeon fanciers fell fourfold over a short interval in the absence of avian exposure and were attributable to antibodies directed against antigens from hen's egg in whi...