Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 ...

One of the fundamental components of large-scale gene discovery projects is that of clustering of Expressed Sequence Tags (ESTs) from complementary DNA (cDNA) clone libraries. Clustering is used to create non-redundant catalogs and indices of these sequences. In particular, clustering of ESTs is frequently used to estimate the number of genes derived from cDNA-based gene discovery efforts. This...

Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free sys...

The preparation of cDNA libraries usually involves multiple steps, such as isolation of poly A mRNA, synthesis of cDNA, cloning of cDNA into plasmid, and amplification of cloned plasmid. The last step, the amplification of insert DNA fragment after cloned in dephosphorylated plasmid vector, is done in either of two ways. The recombinant DNA can be extracted after amplification in host cells, or...

The manuscript by Hong and colleagues in this issue of the American Journal of Respiratory Cell and Molecular Biology (1) profiling the downstream genes activated by expression of the tumor suppressor gene, PTEN , represents one of the first papers published in this journal that makes use of complementary DNA (cDNA) microarrays. cDNA microarray analysis is one of the most important new areas th...

Ochratoxin A (OTA) is positively correlated with an increased risk of developing cancer in nephrotoxic and hepatotoxic patients. Therefore, it great significance for the highly sensitive, selective, timely detection OTA. We described here electrochemical aptasensor OTA analysis, which took advantage favorable properties gold nanoparticles (AuNPs) functionalized zinc oxide (ZnO) composites inter...

Abstract Rapid tests for active SARS-CoV-2 infections rely on reverse transcription polymerase chain reaction (RT-PCR). RT-PCR uses of RNA into complementary DNA (cDNA) and amplification specific (primer probe) targets using (PCR). The technology makes rapid identification the virus possible based sequence homology nucleic acid is much faster than tissue culture or animal cell models. However t...

Complementary DNA (cDNA) microarray is one of the most recent and important technology for exploring the genome. cDNA microarray Image analysis aims to measure the intensity for each spot in the scanned image and this intensity represents the amount of a specific gene in the studied cell. This paper presents a new algorithm that achieves an automated way for applying grid in the cDNA microarray...

The accessibility of specific sequences in domain V of E. coli 23s rRNA in the 50S subunit to complementary oligodeoxyribonucleotides (cDNA) has been investigated. The apparent percentage of subunits engaged in complex formation was determined by incubation of radiolabeled cDNA probe with 50S subunits, followed by nitrocellulose membrane filtration of the reaction mixtures and measurement of th...