The genus Fusarium includes several species that produce trichothecenes. We analyzed DNA sequences from a variable region at the 5' end of the large nuclear ribosomal DNA (rDNA) (28S) to determine the genetic relatedness of trichothecene-producing Fusarium species. All trichothecene-producing strains clustered together, and two monophyletic groups were resolved. The first clade included strains...

ABSTRACT To investigate the interaction between two major ear-rotting pathogens, maize ears were inoculated with either Fusarium graminearum, F. moniliforme, or an equal mixture of the two. Silk and kernel tissues were periodically harvested throughout the growing season so that a time course of the experimental variables (disease severity, ergosterol content, fungal DNA content, and mycotoxin ...

maize genotypes were screened for host resistance and seed treatment chemicals were evaluated in field to manage fusarium ear rot complex of maize at high- and mid-hill environments in nepal during 2003 and 2004. seven popular maize genotypes along with a susceptible check were used in the host resistance study. the maize genotypes adopted from exotic sources, manakamana-3 and deuti, performed ...

Maize is frequently infected by the Fusarium species producing mycotoxins. Numerous investigations have focused on grain maize, but little is known about the Fusarium species in the entire plant used for silage. Furthermore, mycotoxins persist during the ensiling process and thus endanger feed safety. In the current study, we analyzed 20 Swiss silage maize samples from growers' fields for the i...

The objective of this study was to determine the frequencies of distribution and the toxigenic potential of Fusarium species isolated from non-harvested maize left in field over winter in 1999 (N = 56) and 2003 (N = 56) in northern Croatia. Zearalenone and trichothecenes (DON, DAS, T-2) were isolated and detected using multitoxin extraction and TLC method. Modified TLC method was applied to det...

Fusarium infection level, DNA quantity of the Fusarium poae, F. sporotrichioides, F. langsethiae, F. culmorum, F. graminearum and F. equiseti as well as deoxynivalenol (DON), zearalenone (ZEN ) and T-2 toxin (T-2) content were investigated in grain from cultivars of different cereal species grown on organic farming sites during 2005-2006. The Fusarium infection level was examined by agar platin...

Fusarium graminearum Schwabe causes Fusarium head blight (FHB), a devastating disease that leads to extensive yield and quality loss of wheat and other cereal crops. Twelve isolates of F. graminearum were collected from naturally infected spikes of wheat from Assiut Egypt. These isolates were compared using SRAP. The results indicated distinct genetic groups exist within F. graminearum, and dem...

Mycotoxins are secondary metabolites produced by fungi that when ingested at low concentrations are harmful to human and animal health. Toxins from Fusarium graminearum [deoxynivalenol (DON), zearalenone (ZEA)] are commonly found in wheat, barley and corn and their derived products. In Ontario, widespread occurrences of mycotoxins and substantial economic losses have occurred during periodic ep...

Two vomitoxin-producing isolates of Fusarium spp. were grown on cracked corn for 1 to 8 weeks at 15, 20, 25, 28, and 32 degrees C. Maximum production of vomitoxin by Fusarium graminearum Schw. NRRL 5883 occurred at 30 degrees C and 40 days, and that by Fusarium roseum Schw. NRRL 6101 occurred at 26 degrees C and 41 days. These optimum production points were determined from response surface cont...

background: identification and quantification of mycotoxins produced by fusarium species are important in controlling fungal diseases. objectives: potential of zearalenone, butenolide and fusarin c production was investigated in five fusarium graminearum and five f. culmorum isolates at molecular level. materials and methods: presence of pks13, fg08079.1 and pks10 genes, associated with product...