Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

the connection between subject and power is the most important matters in foucault’s contemplation. he wants to configuration the modern subject as a power discourse object by utilize genealogy method and contemplate it in the connection with forms such as discipline technologies. discipline and punishment organized scheme of renewing the power connections network that is disciplined and extend...

In this essay I critically examine Daniel Wegner’s account of conscious will as an illusion developed in his book The Illusion of Conscious Will (MIT Press, 2002). I show that there are unwarranted leaps in his argument, which considerably decrease the empirical plausibility and theoretical adequacy of his account. Moreover, some features essential to our experience of willing, which are relate...

BACKGROUND The ATP7A gene encodes the ATP7A protein, which is a trans-Golgi network copper transporter expressed in the brain and other organs. Mutations in this gene cause disorders of copper metabolism, such as Menkes disease. Here we describe the novel and unusual mutation (p.T1048I) in the ATP7A gene of a child with Menkes disease. The mutation affects a conserved DKTGT1048 phosphorylation ...

quran, the eternal word of god and human capital, since the descent to the present and future generations of muslims will always be special consideration. god's word in a very short time, swept across borders and across wide territories adjacent to their building and conquered the hearts of patriots. this fascination with the muslim holy book, owes much to the aesthetics of the word of god...