A simple, rapid procedure for the presumptive identification of anaerobic bacteria has been evaluated. Two hundred and thirty-five clinical isolates were identified using gas-liquid chromatography and 3-ml volumes of a few selected test media. These test media were stored aerobically and incubated in GasPak anaerobic jars. The average incubation time was 39 h. This procedure, when compared to t...

Anaerobic ammonium oxidation (anammox) involves the microbiological oxidation of ammonium with nitrite as the electron acceptor and dinitrogen gas as the main product. The Scalindua species, an anammox genus that dominates natural habitats, plays an important role in catalysing the loss of nitrogen from marine environments. Until now, a few Scalindua species have been reported to be enriched fr...

It has been observed that Lactobacillus acidophilus has poor survivability in fermented goats’ milk. In this study, interactions between L. acidophilus and starter cultures during goats’ milk fermentation were investigated using three strains of L. acidophilus: ATCC-11975, LA-5 and NCFM, and the starter cultures (Lactobacillus delbrueckii subsp. bulgaricus (LB350) and Streptococcus thermophilus...

A method for semi-micro high-performance liquid chromatography (HPLC) has been established for the simultaneous determination of 3alpha-hydroxyglycyrrhetic acid and 3-dehydroglycyrrhetic acid together with glycyrrhizin, glycyrrhetic acid and glycyrrhetic acid mono-glucuronide formed by incubation of glycyrrhizin with rat feces. The analysis was accomplished within 25 min with a TSKgel ODS-80TsQ...

The introduction of an improved anaerobic system resulted in the isolation of Bacteroides ureolyticus (B corrodens) in numbers that suggested a pathogenic role from many more clinical specimens. During a three-year period B ureolyticus was isolated from 103 fairly superficial necrotic or gangrenous lesions all of which showed evidence of active infection. These included 27 perineal or genital i...

The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxidation to CO2 surpassed methanol assimilation and methanogenesis from CO2/H2 a...

colonies after 48 hours of incubation. The colonies became orange-salmon with prolonged incubation. Anaerobic plate (Schaedler agar) was negative. The organism was a weakly acid-fast grampositive bacillus which also showed rare branching. It was initially identified as an aerobic actinomycete. The confirmation of isolate identity was obtained by sequencing of a fragment of 1,202 bp from 16s rRN...

A microdilution technique using commercially available media and materials was developed and used to determine the minimal inhibitory concentrations (MICs) of clindamycin, chloramphenicol, tetracycline, minocycline, ampicillin, carbenicillin, cephalothin, and gentamicin for 101 anaerobic isolates. Representative strains of Bacteroides, Clostridium, Fusobacterium, Peptococcus, and Peptostreptoco...

The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungus Phanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 degrees C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharif...