Protein-protein interactions (PPI) are involved in a myriad of cellular processes, and their deregulation can lead to many diseases. One such process is protein ubiquitination that requires an orchestrated action three key enzymes add ubiquitin moieties substrate proteins. Importantly, this reversible through deubiquitinating enzymes. Both deubiquitination require PPIs once classified be utiliz...

background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...

The three-dimensional particle-in-cell 3-D PIC simulation code WARP is used to study positron confinement in antihydrogen traps. The magnetic geometry is close to that of a UC Berkeley experiment conducted, with electrons, as part of the ALPHA collaboration W. Bertsche et al., AIP Conf. Proc. 796, 301 2005 . In order to trap antihydrogen atoms, multipole magnetic fields are added to a conventio...

The aim of this study was to investigate the effect of the serine/threonine protein phosphatase inhibitor, calyculin-A (CLA), on clot formation and on the procoagulant activity of human platelets. Platelet-rich plasma (PRP) samples were preincubated with buffer or CLA and subsequently platelets were activated by the protease-activated receptor 1 (PAR-1) activator, thrombin receptor activating p...

This study investigates the ability of larvae and adult rover and sitter Drosophila melanogaster to detect and migrate towards the source of a fly medium attractant using larval plate assays and an adult olfactory trap assay. Allelic variation at the foraging locus which encodes a cGMP-dependent protein kinase (PKG) did not affect larval olfactory response in the larval plate assays. In contras...

The improvement of trap-lure combinations is an important part of integrated pest management programs that involve monitoring pests for timely insecticide applications, or for their use in control strategies such as mass trapping or bait stations. In this study improvements in the capture of Drosophila suzukii were not observed following the inclusion of different color stimuli with respect to ...

The most commonly used optical-trapping assays are coupled to surfaces, yet such assays lack atomic-scale ( approximately 0.1 nm) spatial resolution due to drift between the surface and trap. We used active stabilization techniques to minimize surface motion to 0.1 nm in three dimensions and decrease multiple types of trap laser noise (pointing, intensity, mode, and polarization). As a result, ...

A single-beam gradient-force optical trap for dielectric particles, which relies solely on the radiation pressure force of a TEM(00)-mode laser light, is demonstrated in air for what is believed to be the first time. It was observed that micrometer-sized glass spheres with a refractive index of n=1.45 remained trapped in the focus region for more than 30 min, and we could transfer them three di...