Human pluripotent stem cells (hPSCs) hold significant promise for use in regenerative medicine, or as a model to understand human embryo development. However, the basic mechanisms required for proliferation and self-renewal of hPSCs have not been fully uncovered. Proliferation in all eukaryotes is dependent upon highly regulated expression of the histone H3 variant Centromere protein A (CENP-A)...

Seed development dedicates to reserve synthesis and accumulation and uncovering its genetic and biochemical mechanisms has been a major research focus. Although proteomic and transcriptomic analyses revealed dynamic changes of genes and enzymes involved, the information regarding concomitant metabolic changes is missing. Here we investigated the dynamic metabolic changes along the rice grain de...

A detailed knowledge of storage reserve accumulation in developing seeds can provide vital information to create strategies for improving the lipid content as well as the natural value of castor (Ricinus communis L.) crop. After hand pollination (female flowers-tagged), seed filling (lipid and protein accumulation) in castor bean is initiated as early as 7 days after pollination (DAP). Accumula...

While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profiling to characterize Arabidopsis (Arabidopsis thaliana) seeds throughout developm...

Plant proteins are plausible fishmeal substitutes but are deficient in some essential amino acids (EAA) like lysine and methionine. Combination of different plant proteins with complimentary EAA could be useful alternative. Bambaranut (Voandzeia subterranea) contains high amount of lysine while methionine is in sesame seed (Sesamum indicum). This experiment tested effects of combining sesame se...

Masting, a breeding strategy common in perennial plants, is defined by seed production that is highly variable over years and synchronized at the population level. Resource budget models (RBMs) proposed that masting relies on two processes: (i) the depletion of plant reserves following high fruiting levels, which leads to marked temporal fluctuations in fruiting; and (ii) outcross pollination t...

kermanshah province is the main producer of various chickpea cultivars in iran. in this study a laboratory peg-tooth thresher was employed for chickpea threshing. the effect of cylinder speed (9, 12, 15 m/s), concave clearance (12, 14, 16 mm), feed rate (80, 160, 240 kg/h) and material moisture content (5, 10, 15% w.b.) was studied on percentage of grain damage, threshing efficiency and percent...

A degenerate dual-pump optical parametric chirped-pulse amplifier (OPCPA) for generation of few-cycle intense pulses centered at 1.6 μm is theoretically investigated. By adding the optimized linear chirp to the two pump pulses from Ti:sapphire source and carefully adjusting the delays between the two pumps and seed, the long- and short-wavelength components of the seed pulse are efficiently amp...