PURPOSE Corneal epithelium is maintained by a population of stem cells (SCs) that have not been identified by specific molecular markers. The objective of this study was to find new putative markers for these SCs and to identify associated molecular pathways. METHODS Real time PCR (rt-PCR) was performed in 24 human limbal and central corneal epithelial samples to evaluate the gene expression ...

Corneal epithelium from 5-7-day old chick embryos was isolated with EDTA and grown in culture on frozen-killed lens as a substratum. Autoradiographs showed that in the presence of [(3)H]proline, the corneal epithelium synthesized and secreted onto the lens substratum, radioactive materials resistant to extraction by sodium hydroxide. The radioactive label was associated with newly formed striat...

Ultra-high resolution optical coherence tomography (UHR-OCT) can image the corneal epithelium and Bowman's layer and measurement the thicknesses. The purpose of this study was to validate the diagnostic power of vertical thickness profiles of the corneal epithelium and Bowman's layer imaged by UHR-OCT in the diagnosis of sub-clinical keratoconus (KC). Each eye of 37 KC patients, asymptomatic fe...

tides are seen in the in vivo experiment than in the in vitro one suggests that synthesis of polyglucose is regulated in the cell even under in vivo histochemical conditions. Another use of the enzyme-synthetic approach in the in vivo cornea has been shown in the present study. The number of synthesized polyglucose particles in the corneal epithelium indicates the amount of glucose-1-phosphate ...

The electrical potential difference and electrical resistance of the nonswelling cornea of the dogfish, Squalus acanthias, were examined. It was found that routine procedures used in the procurement of fish invariably produce damage to the corneal epithelium which affects electrical measurements and possibly composition of the aqueous humor. We found no electrical evidence of ionic pumps in the...

For study of corneal adhesion after chemical injury, sulfuric acid was used to burn the central and peripheral corneal epithelium in rhesus monkeys. The adhesion properties of the regenerating epithelium were sequentially followed by clinical assessment and by serial corneal biopsy specimens examined by transmission electron microscopy. The peripheral burns healed uneventfully, but the central ...

Corneal epithelium from 5-7-day old chick embryos was isolated with EDTA and grown in culture on frozen-killed lens as a substratum . Autoradiographs showed that in the presence of [3H]proline, the corneal epithelium synthesized and secreted onto the lens substratum, radioactive materials resistant to extraction by sodium hydroxide . The radioactive label was associated with newly formed striat...

PURPOSE To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithelial cell proliferation. METHODS [3H]-galactose-labeled and unlabeled glyco...

Evidence is presented that the epithelium on a donor corneal allograft is neither sloughed off rapidly in the immediate postoperative period, nor is it even slowly replaced by recipient epithelium in the technically successful corneal transplant. Specific immunologic rejection of the surviving epithelium of a graft may take place long after transplantation, and is characterized by a linear defe...

Acanthamoeba keratitis is a sight-threatening corneal infection. In a recent study, the saccharide mannose has been shown to inhibit the binding of Acanthamoeba organisms to the epithelium of the cornea (L. D. Morton, G. L. McLaughlin, and H. E. Whiteley, Infect. Immun. 59:3819-3822, 1991). In an attempt to determine the molecular mechanism by which acanthamoebae adhere to the surface of the co...