in previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. the aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. separation was achieved on a zorbax...

Since its existent, the principle of the chromatomembrane mass transfer has been used in flow injection analysis mainly for determining of inorganic and organic substances in environmental samples and coupled with several detection systems such as photometry, voltammetry, inductively coupled plasma-atomic emission spectroscopy, gas chromatography, ion chromatography, and high performance liquid...

Nowadays, food contamination by mineral oil (MO) is a very important issue and its presence in edible oils is widely documented. Mineral oils are hydrocarbons mixture of petrochemical origin containing mainly two families of compounds, namely mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH). The most used approach for mineral oil analysis is liquid chromato...

In the present work, a method for the analysis of the residues of chlorpyrifos and its toxic metabolite 3,5,6-trichloro-2-pyridinol (TCP) in agricultural soil and apples was developed. The residues of chlorpyrifos and TCP were extracted from agricultural soil and apple samples by shaking followed clean-up by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) coupled with detection ...

Analytical methods for mycotoxins in cereals and cereal-based products require three major steps, including extraction, clean-up (to eliminate interferences from the extract and concentrate the analyte), and detection/determination of the toxin (by using suitable analytical instruments/technologies). Clean-up is essential for the analysis of mycotoxins at trace levels, and involves the use of s...

The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques. Capillary gas chromatography/mass spectrometry (GC-MS) allows the most efficient separation, identification and quantification of volatile metabolites in biological fluids. Liquid chromatography-mass spectrometry (LC-MS) is especially suitable for the analysis of non-volatile...

A rapid method of microwave-assisted extraction coupled with high-speed counter current chromatography and UV detector was established for the preparation of phytosterols from edible marine algae. Extraction conditions, namely microwave power, liquid/solid ratio, irradiation time and extraction temperature were optimized using orthogonal array design. The microwave-assisted extraction of Undari...

Submit Manuscript | http://medcraveonline.com Abbreviations: ANVISA: National Health Surveillance Agency; BHA: mixture of 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl4-hydroxyanisole; BHT: 3,5-di-tert-butyl-4-hydroxytoluene; CGMS: Gaseous Chromatography coupled to Mass Spectrometry; HPLC: High Pressure Liquid Chromatography; PG: Propyl 3,4,5-trihydroxybenzoate or Propyl Gallate; TBHQ: Tertbut...

Safe use of cyclosporine (CsA) in solid organ transplantation relies on regular whole-blood drug monitoring. Several promising immmunoassays, e.g. the antibody-conjugated magnetic immunoassay (ACMIA) method, were developed and commercialized during recent years to compete with liquid chromatography coupled to tandem mass spectrometry, which remains the reference method but is labor-intensive. W...

A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of...