The nucleotide sequence of a 869 bp segment of phage 434 DNA including the regulatory genes cro and cII is presented and compared with the corresponding part of the phage lambda DNA sequence. The 434 cro protein as deduced from the DNA sequence is a highly basic protein of 71 amino acid residues with a calculated molecular weight of 8089. While the cro gene sequences of phage 434 and lambda DNA...

BACKGROUND Antimicrobial resistance of Neisseria gonorrhoeae is a serious health problem in China. Gonococcal antimicrobial susceptibility has been monitored in Shanghai since 1988. In this study, we examined the changing pattern of gonococcal antimicrobial susceptibility based on data from N. gonorrhoeae isolates collected over the past 25 years. METHODS Approximately 100-200 isolates each y...

The phage lambda transcription antitermination protein, pN, acts with host factors, Nus, at sites on the phage genome, nut, to render RNA polymerase resistant to subsequent downstream termination signals. The NusA protein appears to recognize a seven to eight base-pair consensus sequence (5'Py-G-C-T-C-T-T(T)3') called boxA that is found in the promoter-proximal part of the nut region. Two types...

The mammalian gut is a dynamic community of symbiotic microbes that interact with the host to impact health, disease, and metabolism. We constructed engineered bacteria that survive in the mammalian gut and sense, remember, and report on their experiences. Based on previous genetic memory systems, we constructed a two-part system with a "trigger element" in which the lambda Cro gene is transcri...

Using a module exchange approach, we have tested a longstanding model for the role of Cro repressor in prophage induction. This epigenetic switch from lysogeny to the lytic state occurs on activation of the host SOS system, which leads to specific cleavage of CI repressor. It has been proposed that Cro repressor, which operates during lytic growth and which we shall term the lytic repressor, is...

The optimal reactive power flow (ORPF) helps to effectively utilize the existing reactive power sources for minimizing the network loss. The chemical reaction optimization (CRO), inspired from the interactions of molecules in a chemical reaction to reach a low energy stable state and searches for optimal solution through reactions involving the on-wall ineffective collisions, decomposition, int...

The precise duration of therapy of multidrug-resistant (MDR) typhoid with broad-spectrum cephalosporins is uncertain. We prospectively randomized 57 children with culture-proven MDR typhoid to receive treatment with intravenous ceftriaxone (CRO) (65 mg/kg of body weight/day) for 7 days (short course; n = 29) or 14 days (conventional; n = 28). The response to therapy, as evaluated by the serial ...

Este artigo discute alguns problemas argumentativos de recentes publicações relacionadas à pandemia do Covid-19 feitas pelo CFO e CRO-GO, CRO-RS CRO-MG. A presente análise tem por objetivo instigar a reflexão crítica sobre questões científicas, jurídicas morais pujantes na pandemia, especialmente (mas não apenas) daqueles que têm obrigação (moral jurídica) promover ética entre seus inscritos. I...

This paper is an essay to connect with the stone Age coastal hunters who sheltered 28,000 years ago in the caves of today's italian Mediterranean sea shore cliffs. We have focused on the archaeological Gravettian layer in the Cavillon cave from Grimaldi occupied by Cro Magnons (informal name for Anatomically Modern Humans of the European Upper Paleolithic), which demonstrates the technical skil...

The typical smelting wastewater is with characteristics of high concentration TDS, conductivity, heavy metal ions, and low COD. ultrafiltration (UF) reverse osmosis (RO) an attractive technology to reclaim reuse this kind wastewater. In work, Coagulation-flocculation-sedimentation (CFS) multi-media filter (MMF) was used as the pretreatment processes. UF, RO (CRO) processes applied advanced trea...