BACKGROUND/OBJECTIVE Blood bank audit embraces all procedures from blood procurement to the long -term consequences of transfusion which helps to identify opportunities for improvement. We have therefore looked at red cell transfusion in our hospital to identify areas that will require improvement. METHOD Data was collected retrospectively from the hard copy record of the blood bank for a per...

Alloimmunization to donor class I HLA antigens represents a major obstacle to successful platelet transfusion therapy. It is desirable to distinguish alloimmunization from nonimmunologic causes of poor platelet survival to assess the need for HLA-matched, single-donor platelets. We describe a new in vitro assay for anti-HLA antibodies and report its application to the problem of platelet crossm...

IntroductionAllosensitization presents a barrier to lung transplantation in otherwise acceptable patient candidates leading increased waitlist times and mortality. We present case of highly sensitized dual organ kidney-lung transplant recipient that did not develop AMR despite from positive crossmatch donor after treatment with an Eculizumab based protocol.Case ReportOur is 57-year-old woman id...

Clinical studies have demonstrated that HLA-DP-specific antibodies can be detrimental to a transplanted kidney. The number of patients affected is proportional to the frequency of DP antibodies. We determined the frequency of HLA-DP-specific antibodies en toto and in the absence of cross-reactive DR antibodies. Of 650 waitlisted renal patients, 271 (42%) were reactive with HLA-DP antigens in so...

Antibodies directed against HLA antigens of a given organ donor represent the dominating reason for hyper-acute or acute allograft rejections. In order to select recipients without donor-specific antibodies, a standard crossmatch (CM) procedure, the complement-dependent cytotoxicity assay (CDC), was developed. This functional assay strongly depends on the availability of isolated vital lymphocy...

111In-labeled granulocytes were used to study the effects of histocompatibility factors on the migration of transfused granulocytes to infected sites. Fourteen alloimmunized and 20 nonalloimmunized patients received approximately 10(8) 111In-labeled granulocytes from ABO-compatible, non-HLA-matched donors, and scans were performed over known infected sites. All 14 alloimmunized patients had lym...

The requirements of pretransfusion testing have undergone repeated modification, and those of the type and screen policy are currently the most widely accepted to be standard. The type and screen test policy, together with the abbreviated crossmatch procedure, was implemented in the United Christian Hospital in January 1997. This paper discusses the impact of the type and screen test policy on ...

BACKGROUND Prognosis of renal transplants with positive flow cytometric crossmatch (FCXM) remains controversial. METHODS We analyzed the outcome of these kidney transplant recipients by human leukocyte antigen (HLA) donor-specific antibodies (HLA-DSA) using single-antigen bead (SAB) assays in major histocompatibility complex classes I and II. We compared them with controls with a negative FCX...