This study was designed to examine the stage dependency of growth inhibition by Streptomyces sp. crude extract and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by differences in growth appearance between treated and control parasites. Using synchronized In vitro cultures of Plasmodium falciparum strain 3D7, growth inhibition was detec...

two experiments were conducted to evaluate the crude protein fractionation, in vitro gas production and the biological effects of tannins in alfalfa silage treated with different levels of pistachio by-products (pb) extract. pb was soaked in water with a ratio of 1:5 (w/v) for 12 h. after filtering and concentrating, the crude extract was added to fresh alfalfa to a final concentration of 1%, 2...

The crude methanol extract of the seeds of Derris trifoliata showed potent and dose dependent larvicidal activity against the 2nd instar larvae of Aedes aegypti. From this extract two unusual rotenoid derivatives, a rotenoloid (named 7a-O-methyl-12a-hydroxydeguelol) and a spirohomooxarotenoid (named spiro-13-homo-13-oxaelliptone), were isolated and characterised. In addition a rare natural chro...

This study evaluated the antimicrobial activity of the crude ethanol extract and fractions from Eugenia uniflora L. leaves against Pseudomonas aeruginosa. A total of 72 P. aeruginosa isolated from patients of three hospitals in Goiânia and 8 standard strains were selected to test antimicrobial activity. The bacteria susceptibility profile against 15 antimicrobial agents was determined using the...

Monoclonal antibodies were obtained after fusion of mouse myeloma cells with spleen cells isolated from mice primed with a crude extract of yeast killer toxin produced by a strain of Hansenula anomala. Hybridomas were selected by specific immunoassay reaction of their fluid with crude yeast killer toxin extract. Among the monoclonal antibodies, which were characterized by the Western blot techn...

Among sixty one yeast isolates from various samples, twelve were capable of producing phytase. Out of twelve, 5 isolates showed maximum phytase activity. CB3 isolate was selected for further study. CB3 isolate was genetically characterized as Candida parapsilosis. The crude extract was optimized for maximum enzyme activity. The enzyme was stable between the pH 2 to 7 but the optimal pH was foun...

The biodegradation effect and mechanism of decabromodiphenyl ether (BDE-209) by crude enzyme extract from Pseudomonas aeruginosa were investigated. The results demonstrated that crude enzyme extract exhibited obviously higher degradation efficiency and shorter biodegradation time than Pseudomonas aeruginosa itself. Under the optimum conditions of pH 9.0, 35 °C and protein content of 2000 mg/L, ...