Recent studies illustrate the potential for improving the cryopreservation of stem cells. Reduced DMSO concentrations in the cryopreservation medium, post thaw washing of cells and increased cell concentration have been actively studied. Standardization of cell processing has led to the study of liquid storage prior to cryopreservation, validation of mechanical (uncontrolled rate freezing) free...

Cellular therapy with stem and immune cells has demonstrated significant success both in clinical treatments and the industrial market. Cryopreservation is a necessary and essential component of cellular therapy. In this chapter, first of all, some basic theories of cryoinjury and techniques in cryopreservation are reviewed. Then it focuses on the progress of cryopreservation of stem cells and ...

Diploidic germplasms such as embryos, compared to haploidic gametes, are theoretically a better choice for preservation of an animal species. However, there are significant challenges in cryopreservation of multicellular materials due to their size and physical complexity which affect the permeation of cryoprotectants and water, sensitivity to chilling and toxicity of cryoprotectants. While cry...

OBJECTIVE To review historical and contemporary advances in oocyte-cryopreservation techniques and outcomes. DESIGN Publications related to oocyte cryopreservation were identified through MEDLINE and other bibliographic databases. CONCLUSION(S) Oocyte cryopreservation can be used as an adjunct to conventional IVF and as an option for fertile women to electively cryopreserve their gametes. R...

Gamete cryopreservation combined with in vitro embryo production (IVP) and cryopreservation of resultant embryos are important technologies for gene banking in domestic animals. Especially, cryopreservation of embryos and oocytes offers the possibility for the preservation of female genetic lines. Although several vitrification protocols have been developed for the cryopreservation of embryos a...

BACKGROUND Despite interest in cryopreservation of individual or small number of human spermatozoa, to date, little data is available as regards its effectiveness. We systematically reviewed the outcome after cryopreservation of individual or small numbers of human spermatozoa in patients with severe male factor of infertility. METHODS We searched the MEDLINE, EMBASE, Cochrane Systematic Revi...

The value of preserving high quality bio specimens for fundamental research is significant as linking cellular and molecular changes to clinical and epidemiological data has fueled many recent advances in medicine. Unfortunately, storage of traditional biospecimens is limited to fixed samples or isolated genetic material. Here, we report the effect of cryopreservation of routine myocardial biop...

Ovarian tissue cryopreservation is an option to preserve reproductive potential in patients who must urgently undergo aggressive chemotherapy and/or radiotherapy or who have other medical conditions requiring treatment that may threaten ovarian function and subsequent fertility. Ovarian tissue cryopreservation may be the only option available to prepubertal girls undergoing such treatments. How...

This experiment was designed to compare powdered egg yolk with fresh egg yolk in an extender for cryopreservation of Zebu bull semen. Sperm motility, plasma membrane integrity and viability were assessed at different stages of cryopreservation (post-dilution, pre-freezing and post-thawing). Sperm plasma membrane integrity remained similar at all the stages of cryopreservation. Sperm motility an...

INTRODUCTION The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of rabies virus using different cryopreser...