Mycoplasma gallisepticum is the most important and infectious Mycoplasmosis. It is caused lots of economic losses for poultry's industry of Iran. The target of this study is comparison of culture and nested PCR techniques to detect Mycoplasma gallisepticum infection of chicken’s from industrial farms in Kerman province of Iran. 88 isolates received from industrial far...

background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...

purpose: to compare the value of conventional laboratory methods and polymerase chain reaction (pcr) in the diagnosis of fungal keratitis. methods: this cross sectional study was conducted at khalili hospital, shiraz, iran. samples were taken from thirty-eight patients with findings suspicious for fungal keratitis. corneal scrapings were used for gram, giemsa and koh stains, culture and pcr ana...

background and objective: rapid diagnosis of pertussis is important for the timely isolation of the infection source and early prevention measures among the contact persons, especially among non-vaccinated infants for whom pertussis is life- threatening. materials and methods: targets is481 , is1001, bp0026 and human gapdh gene were used to develop a multiplex real- time pcr assay based on the ...

BACKGROUND Pathogenic Leptospira spp. present in the blood of patients with leptospirosis during the first week of symptoms can be detected using culture or PCR. A proportion of patients who are positive by PCR are negative by culture. Leptospira spp. are fastidious bacteria, and we hypothesized that a false-negative culture result may represent infection with a distinct bacterial subset that f...

BACKGROUND The aims of this study were to compare several DNA extraction methods and 16S rDNA primers and to evaluate the clinical utility of broad-range PCR in continuous ambulatory peritoneal dialysis (CAPD) culture fluids. METHODS Six type strains were used as model organisms in dilutions from 10(8) to 10(0) colony-forming units (CFU)/mL for the evaluation of 5 DNA extraction methods and 5...

Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI – followed by PCR of lymph node aspirate culture –...

BACKGROUND Cytomegalovirus (CMV) infection is the most common cause of congenital infection. Whereas CMV PCR has replaced viral culture and antigen detection in immunocompromised patients because of higher sensitivity, viral culture of neonatal urine is still referred to as the gold standard in the diagnosis of congenital CMV infection. OBJECTIVE To compare real-time CMV PCR with shell vial c...