Cyclooxygenase (COX-1/COX-2)-catalyzed eicosanoid formation plays a key role in inflammation-associated diseases. Natural forms of vitamin E are recently shown to be metabolized to long-chain carboxychromanols and their sulfated counterparts. Here we find that vitamin E forms differentially inhibit COX-2-catalyzed prostaglandin E(2) in IL-1beta-stimulated A549 cells without affecting COX-2 expr...

We aimed at evaluating the relative contribution of cyclooxygenase (COX) -1 and COX-2 to the synthesis of prostacyclin in endothelial cells under static conditions in the presence or absence of exogenous arachidonic acid and/or altered intracellular redox balance. Selective inhibitors of either COX-1 (SC560 and FR122047) or COX-2 (SC236) concentration dependently (1-300 nM) reduced basal and in...

Two compounds (celecoxib and valdecoxib) from the diarylheterocycle class of cyclooxygenase inhibitors were radiolabeled and used to characterize their binding to cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), several single-point variants of COX-2 (Val523Ile, Tyr355Ala, Arg120Ala, Arg120Gln, Arg120Asn) and one triple-point variant of COX-2 [Val523Ile, Arg513His, Val434Ile (IHI)]. We demon...

OF DISSERTATION Rattanavijit Vijitruth The Graduate School University of Kentucky 2006 ROLES OF CYCLOOXYGENASE-2 IN MICROGLIAL ACTIVATION AND DOPAMINERGIC CELL DEATH A dissertation submitted in partial fulfillment of the requirement for the degree of Doctor of Philosophy in the College of Medicine at the University of Kentucky By Rattanavijit Vijitruth Lexington, Kentucky Director: Dr. Guoying ...

Endothelial Nitric Oxide Synthase, Cyclooxygenase-2, and Essential Hypertension: Is There an Interaction? To the Editor: Nitric oxide (NO), prostacyclin (PGI2), and thromboxane (TxA2) are produced by NO synthase (NOS) and cyclooxygenase (ie, COX-1 and COX-2) isoforms, respectively. They are highly potent vasoactive molecules. NOS and COX pathways are assumed to interact and modulate each other....

PURPOSE Cyclooxygenase-2 (COX-2), one isoform of cyclooxygenase proinflammatory enzymes, plays an important role in tumor development and progression. Researches of human cancers have revealed high expression levels of COX-2 in a variety of cancers including lung cancer. The mechanism of COX-2 in the pathogenesis of non-small cell lung cancer (NSCLC) cells is not well understood. METHODS We c...