The bacmid system of BmMNPV with cysteine protease gene deletion (CPD-BmMNPV bacmid) was constructed using the lambda recombination system. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid were reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein was...

Thougha numberof proteinaceous cysteine proteinase inhibitors (cystatins) of animal origin have long been studied in detail,1* little is well-defined about plant cystatins except for oryzacystatins from rice seeds.2'3) Preceding the studies on oryzacystatins, however, we purified a cysteine proteinase inhibitor from corn.4'5) In this study we carried out amino acid analysis and kinetic studies ...

We have identified and characterized three stage-specific proteinases of Plasmodium falciparum that are active at neutral pH. We analyzed ring-, trophozoite-, schizont-, and merozoite-stage parasites by gelatin substrate PAGE and characterized the identified proteinases with class-specific proteinase inhibitors. No proteinase activity was detected with rings. Trophozoites had a 28 kD proteinase...

BACKGROUND A recent report demonstrated the immunogenic character of the cysteine proteinases of Trichomonas vaginalis. It was of interest, therefore, to examine for the presence of serum anti-proteinase antibody among patients with trichomoniasis. METHODS An immunoprecipitation assay was used involving protein A-bearing Staphylococcus aureus first coated with the IgG fraction of goat anti-hu...

In order to access the role of the Porphyromonas gingivalis Arg-gingipain proteases in the virulence of this organism, a mutant defective in the rgpA gene was constructed in strain 381. This mutant, MT10, displayed only 40% of the Arg-specific cysteine protease activity of the wild-type strain. In addition, MT10, as well as the recently characterized protease mutant G-102, which is defective in...

The present study aimed to investigate, as a follow-up of microarray profiling, the expression of the lysosomal cysteine protease cathepsin S and that of its endogenous inhibitor cystatin C in the most common primary intraocular tumor in adults, uveal melanoma. The expression pattern unveiled was characterized by a relative increase in the active form of the elastolytic and collagenolytic cathe...

Activity of the cysteine adducts of the cysteine proteinases papain and thaumatopain can be recovered by treatment with thioredoxin, thioredoxin reductase and NADPH. Recovery of proteinase activity did not occur if any of the components of the thioredoxin system were omitted, or if thioredoxin or thioredoxin reductase were heat-inactivated. Such an enzyme-mediated process may be of significance...

Organic ultraviolet filters (OUVFs) and nanoplastics (NPs) are two important new pollutants that coexist widely in freshwater coastal habitats. Both OUVFs MPs can be taken up by aquatic organisms may toxic or co-toxic. In this study, the neurotoxicity interaction of typical butyl methoxydibenzoyl methane (BM-DBM) polystyrene (PS-NPs) on zebrafish at environmental concentrations were comprehensi...

The pineapple waste from the industry has contributed to an increase in Malaysia and worldwide every year. A major type of endopeptidase enzymes found is fruit bromelain, stem ananain, comasain. This study aims extract purify protease crown MD2 pineapple. Protease was extracted purified using anion exchange chromatography, gel filtration, desalting before being identified liquid chromatography-...