The process of normal bone remodeling requires the continual resorption and replacement of bone mineral and bone matrix proteins. Excessive bone resorption is a feature of a number of pathological conditions. These include osteoporosis, rheumatoid arthritis and Paget's disease [l]. Bone resorption is accomplished by the osteoclast. Osteoclasts attached to bone are highly polarised cells [2]. Th...

Inhibitors of cysteine proteinases have been used extensively to dissect the roles of these proteinases in cells. Surprisingly though, little work has been performed to demonstrate unequivocally that the inhibitors reach and inactivate their target proteinases in cell culture or in vivo. In the present study, the permeability of lysosomes and whole cells has been studied. Benzyloxycarbonyl (Z)-...

background : trichomonas vaginalis is a pathogenic protozoon and may be contaminated with dsrna virus called trichomonas vaginalis virus (tvv). the viral infection is an important factor for its patho-genesis and sensitivity to metronidazole. the presence of tvv is associated with qualitative and quantitative expression of cysteine proteinases and surface immunogenic; p270. the purpose of this ...

The ability of Entamoeba histolytica trophozoites to destroy monolayers of baby hamster kidney cells is inhibited by allicin, one of the active principles of garlic. Cysteine proteinases, an important contributor to amebic virulence, as well as alcohol dehydrogenase, are strongly inhibited by allicin.

Endogenous inhibitors tightly control the activity of proteinases in the extracellular space. Proteinase/antiproteinase imbalance may be caused by predominance of proteinases, resulting in severe tissue damage or abundance of proteinase inhibitors, leading to a shift in the balance of synthesis and degradation of extracellular matrix proteins and accumulation of these matrix components. Lung fi...

The major proteinase activity in extracts of larval midguts from the southern corn rootworm (SCR), Diabrotica undecimpunctata howardi, was identified as a cysteine proteinase that prefers substrates containing an arginine residue in the P1 position. Gelatin-zymogram analysis of the midgut proteinases indicated that the artificial diet-fed SCR, corn root-fed SCR, and root-fed western corn rootwo...

Cysteine proteinases (thiol) carry out diverse and critical functions in plants through their ability to hydrolyze peptide bonds target proteins. Here, we cloned a cysteine proteinase gene designated VaCP17 from highly cold-resistant wild Vitis amurensis accession ‘Shuangyou’, then its potential function cold resistance was investigated. The results showed that the CDS of is 1404 bp, encoding 4...

The interaction between recombinant human cystatin C and the cysteine proteinases papain and actinidin was studied by spectroscopic, kinetic and equilibrium methods. The absorption, near-u.v.c.d. and fluorescence-emission difference spectra for the cystatin C-proteinase interactions were all found to be similar to the corresponding spectra for chicken cystatin. The kinetics of binding of cystat...

To analyze the degradation system in epidermal cells during their generation, differentiation, and cell death, immunocytochemical localization of lysosomal cysteine and aspartic proteinases, an endogenous cysteine proteinase inhibitor, cystatin beta, and ubiquitin were examined using rat sole skin. By confocal laser microscopy, granular immunodeposits for lysosomal proteinases were well demonst...