introduction: intracellular ph (phi) regulates essentially all aspects of cellular activities. however, it is unknown how endoplasmic reticulum (er) potassium channels sense phi. in this study, we investigate the direct effects of phi on er potassium channels. methods: we used channel incorporation into the bilayer lipid membrane method. l-α-phosphatidylcholine, a membrane lipid, was extracted ...

background: gonadotropin-releasing hormone agonists or antagonists are used in assisted reproductive technique cycles as premature luteinizing hormone secretion inhibition. studies have been reported different and contradictory results on the serum progesterone effect on intra-cytoplasmic sperm injection. objective: the purpose of this study was to evaluate the effect of serum progesterone leve...

exploitation of the full potential of any hybrid requires the possessing of genetically high-purity seeds. commercial soybean hybrids have been developed using a cytoplasmic male sterility (cms) system. in order to avoid reduction in yield caused by using low-purity seeds, development of a simple, rapid, and accurate method for hybrid purity assessment is of great essence and significance. ther...

The plasma membrane of Listeria monocytogenes strain 42 was prepared by osmotic lysis of protoplasts with tris(hydroxymethyl)aminomethane (Tris) buffer, pH 8.2, containing MgCl(2) and glucose, followed by washing with NaCl and MgCl(2) in Tris buffer. Electron microscopy showed that the preparation was not contaminated with cytoplasmic material. The membrane preparation was composed of 55 to 60%...

fasting induces short-term physiological adaptations which spare the body's remaining carbohydrate stores and mobilize lipid stores to provide a substitute fuel for many tissues and organs, especially skeletal muscle. rodent studies show that regular occurrence of fasting then refeeding, stimulates adaptations in muscle which make the animal better placed to withstand a further period of fastin...

abstract   aluminum is one of the most important heavy metals which not only can be easily absorbed by roots, but it also damages its normal function and blocks absorption of water and nutrients. according to this fact that sunflower has been widely used in industry, this study investigates the effects of aluminum toxicity on biochemical factors in two sunflower varieties including sirena (tole...

football participation requires considerable utilization of both aerobic and anaerobic energy systems to match the high energetic demands of the sport. the consequent stresses imposed on the physiological and metabolic systems carries players to the threshold of exhaustion during match-play, from which they are required to recover in preparation for the subsequent game. a high number of players...

background: allergens are mostly composed of glycoprotein structures. it is believed that glycan-specific antibodies may lead to false-positive reactions in immunoassays. in this study we investigated the glycosylation state of grape allergens as well as the presence of antibodies to cross-reactive carbohydrate determinants (anti-ccds) in sera from grape-sensitive individuals. methods: grape ex...

the possibility of occurrence of some rearrangements inside and or around coxii and atp6 genes and their relationship with male sterility in rice lines having wild abortive (wa)  cytoplasm was studied. two sterile (ir58025a and ir62829a) and two maintainer lines (ir58025b and ir62829b) were used. radioactive southern blotting was employed to carry out the experiments. the hybridization of coxii...

methods s. cerevisiae was cultured and then disrupted by sonication. after centrifugation, the harvested supernatant was considered to be a cytoplasmic extract. the protein concentration was determined by the biuret method and the extract was diluted to concentrations of 500, 1000, and 2000 µg protein/ml. the frequencies of apoptosis and necrosis were assessed in extract-treated k562 cells by e...