The purposes of this study were to develop an efficient method of labeling D-glucosamine hydrochloride with gallium 68 (68Ga) and investigate the imaging properties of the resulting radiotracer in a human tumor xenograft model using micro-positron emission tomography (μPET). The precursor compound 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-2-deoxy-D-glucosamine (DOTA-DG) wa...

~-Amino-~,6-dideoxy-~-galactosc has been identified as a component of the wall of Pseudumonas JIuorescens (NcTC10038). This amino sugar was present in the lipopolysaccharide fraction of the wall : other amino compounds present were glucosamine, galactosamine, quinovosamine, alanine and ethanolamine. The distribution of amino sugars within different regions of the lipopolysaccharide was studied ...

Chitooligosaccharides (COS) are homo- or hetero-oligomers of D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) that can be obtained by chitosan chitin hydrolysis. Their enzymatic production is preferred over other methodologies (physical, chemical, etc.) due to the mild conditions required, fewer amounts waste its efficiency control product composition. By properly selecting enzyme (chit...

The lipid A component of lipopolysaccharides from Fusobacterium nucleatum Fev 1 consists of beta-1',6-linked D-glucosamine disaccharides, which carry two phosphate groups: one in glycosidic and one in ester linkage. The amino groups of the glucosamine disaccharides are substituted by D-3-hydroxyhexadecanoic acid. The hydroxyl groups of the disaccharide backbone are acylated by tetradecanoic, he...

cystic hydatid disease is caused by cystic larval (metacestode) stages of echinococcus granulosus. this parasite can potentially occur all over the world especially in mediterranean and middle east countries and some parts of africa, latin america and china which have major foci of human infections. the cyst wall of metacestodes consists of inner, middle and external layers. to date, little att...

Glycans of cell surface glycoproteins are involved in the regulation of cell migration, growth, and differentiation. N-acetyl-glucosaminyltransferase V (GnT-V) transfers N-acetyl-d-glucosamine to form β1,6-branched N-glycans, thus playing a crucial role in the biosynthesis of glycoproteins. This study reveals the distinct expression of GnT-V in STRO-1 and CD-146 double-positive dental pulp stem...

The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic oscillation. The antigen is largely destroyed when extracted from whole cells i...

A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contaminatio...

Cell suspensions of Micrococcus sodonensis (ATCC 11880) secrete 7 to 10 individual proteins including an alkaline phosphatase, a nuclease, and a protease. The appearance of enzyme activities in the extracellular medium is dependent on the co-secretion of at least one of several polysaccharides also elaborated by these cells. This conclusion is based on the following observations: (a) bacitracin...