AIM To investigate the influence of refractive index of aqueous humour on imaging of corneal endothelium in confocal microscopy. To clarify the phenomenon of dark endothelial and bright epithelial cell membranes in confocal images of corneas. METHODS Use of a novel digital confocal laser scanning microscope, a combination of the Heidelberg retina tomograph (HRT II) and the Rostock cornea modu...

This study aims to investigate the occurrence and nature of dark neurons in the central nervous system under physiological conditions. Mouse brain tissues were perfusion-fixed with paraformaldehyde or glutaraldehyde at 4 h intervals during one day (3:00, 7:00, 11:00, 15:00, 19:00, 23:00). Paraffin sections were stained with the cationic colloidal iron method, and counterstained with nuclear fas...

Cell-suspension cultures of Lithospermum erythrorhizon Sieb. et Zucc. (Boraginaceae) grown in the dark produce a large amount of red naphthoquinone pigments that are shikonin derivatives when transferred to the production medium M9 (Fujita et al., 1981) from the Linsmaier Skoog liquid medium (Linsmaier and Skoog, 1965). It is known that shikonin biosynthesis is completely inhibited by irradiati...

[Brief letters to the Editor that make specific scientific reference to papers published previously in THE JOURNAL OF GENERAL PHYSIOLOGY are invited. Receipt of such letters will be acknowledged, and those containing pertinent scientific comments and scientific criticisms will be published.] Antagonistic Process as Source of Visible-Light Suppression of Afterpotential in Limulus UV Photorecepto...

This paper presents the characterization and analysis of a 16.8%-efficient 18 um silicon solar cell on steel substrate. Photoluminescence (PL) is used to monitor the solar cell’s fabrication step by step, and a correlation between PL intensity and implied open circuit voltage is established. PL images yield spatially resolved maps of key electrical parameters such as dark reverse saturation cur...

Most of the matter in the universe is invisible. I review the status of dark matter and describe how both the theory of galaxy formation and novel types of experimental searches are revitalizing attempts to find non-baryonic dark matter.

: Optical separate spatial dark and bright soliton pairs in steady-state case in one dimension, for a series circuit consisting of two-photon photorefractive (PR) crystal are investigated. Each crystal can be supported the spatial soliton, and at least one must be photovoltaic. The two solitons are known collectively as separate spatial soliton pairs with dark–dark, bright–dark and bright–brigh...

The characteristic motility of cholera vibrios, as viewed through a dark-field microscope, and the adhesiveness of chicken cell-positive vibrios provide a means for rapidly identifying and biotyping cholera vibrios. Dilute suspensions of vibrios, such as one might find in a fresh rectal swab specimen from a cholera patient, when mixed with a 0.25% suspension of chicken erythrocytes in saline, c...