1. A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of ...

The proportion of sheared rat liver DNA recovered from benzoylated DEAE-cellulose in the final stage following stepwise elution with NaCl and caffeine solutions was dependent upon the DNA isolation procedure. An increase in the proportion of DNA containing single stranded regions, consequent upon delay or addition of Mg2+ prior to phenol extraction, suggested nuclease mediated degradation. Admi...

Olivier Cassar, Sylviane Bassot, Sabine Plancoulaine, Lluis Quintana-Murci, Christine Harmant, Vladimir Gurtsevitch, Natalia B. Senyuta, Larissa S. Yakovleva, Guy de Thé, and Antoine Gessain Author affi liations: Institut Pasteur, Paris, France (O. Cassar, S. Bassot, S. Plancoulaine, L. Quintana-Murci, G. de Thé, C. Harmant, A. Gessain); Institut National de la Santé et de la Recherche Médicale...

Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate compositio...

Ion-exchange chromatography (IEC) using commercial ionic absorbents is a widely used technique for protein purification. Protein adsorption onto ion-exchange adsorbents often involves a multipoint adsorption. In IEC of multimeric proteins or "soft" proteins, the intense multipoint binding would make the further desorption difficult, even lead to the destruction of protein structure and the loss...

Growth rate of heifers is reduced by prostaglandin F2 alpha (PGF) immunization following a primary and booster regimen. The objective was to attenuate the immune response with or without a booster immunization; specifically, the effects of booster interval, dose of PGF-human serum albumin (HSA) conjugate at booster, adjuvant type, or single immunization with one or two adjuvants were examined. ...